Over the years, protein engineers have studied nature and borrowed its tricks to accelerate
protein evolution in the test tube. While there have been considerable advances, our ability …

Light is essential for various biochemical processes in all domains of life. In its presence
certain proteins inside a cell are excited, which either stimulates or inhibits subsequent …

Insertions and deletions (indels) enable evolution and cause disease. Due to technical
challenges, indels are left out of most mutational scans, limiting our understanding of them in …

A long-standing goal in protein science and clinical genetics is to develop quantitative
models of sequence, structure, and function relationships to understand how mutations …

Protein domains are the basic units of protein structure and function. Comparative analysis
of genomes and proteomes showed that domain recombination is a main driver of …

Optogenetics harnesses natural photoreceptors to non‐invasively control selected
processes in cells with previously unmet spatiotemporal precision. Linking the activity of a …

Point-of-care sensors, which are low-cost and user-friendly, play a crucial role in precision
medicine by providing quick results for individuals. Here, we transform the conventional …

Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use
remains limited because the already intricate split site identification problem is often …

Abstract Domain insertion engineering is a promising approach to recombine the functions
of evolutionarily unrelated proteins. Insertion of light‐switchable receptor domains into a …

The multiheme cytochrome MtrA enables microbial respiration by transferring electrons
across the outer membrane to extracellular electron acceptors. While structural studies have …