Engineering compartmentalized biomimetic micro-and nanocontainers

T Trantidou, M Friddin, Y Elani, NJ Brooks, RV Law… - Acs Nano, 2017 - ACS Publications
Compartmentalization of biological content and function is a key architectural feature in
biology, where membrane bound micro-and nanocompartments are used for performing a …

Building reconfigurable devices using complex liquid–fluid interfaces

J Forth, PY Kim, G Xie, X Liu, BA Helms… - Advanced …, 2019 - Wiley Online Library
Liquid–fluid interfaces provide a platform both for structuring liquids into complex shapes
and assembling dimensionally confined, functional nanomaterials. Historically, attention in …

Spontaneous curvature, differential stress, and bending modulus of asymmetric lipid membranes

A Hossein, M Deserno - Biophysical Journal, 2020 - cell.com
Lipid bilayers can exhibit asymmetric states, in which the physical characteristics of one
leaflet differ from those of the other. This most visibly manifests in a different lipid …

Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density

J Steinkühler, E Sezgin, I Urbančič, C Eggeling… - Communications …, 2019 - nature.com
Regulation of plasma membrane curvature and composition governs essential cellular
processes. The material property of bending rigidity describes the energetic cost of …

Reconfigurable printed liquids

J Forth, X Liu, J Hasnain, A Toor, K Miszta… - Advanced …, 2018 - Wiley Online Library
Liquids lack the spatial order required for advanced functionality. Interfacial assemblies of
colloids, however, can be used to shape liquids into complex, 3D objects, simultaneously …

Optimization of the inverted emulsion method for high‐yield production of biomimetic giant unilamellar vesicles

A Moga, N Yandrapalli, R Dimova… - ChemBioChem, 2019 - Wiley Online Library
In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the
construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's …

Light-triggered enzymatic reactions in nested vesicle reactors

JW Hindley, Y Elani, CM McGilvery, S Ali… - Nature …, 2018 - nature.com
Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and
in bottom-up synthetic biology as chassis for artificial cells. In both these areas the …

Exploring giant unilamellar vesicle production for artificial cells—Current challenges and future directions

L Van de Cauter, L van Buren, GH Koenderink… - Small …, 2023 - Wiley Online Library
Creating an artificial cell from the bottom up is a long‐standing challenge and, while
significant progress has been made, the full realization of this goal remains elusive …

Bending rigidity, capacitance, and shear viscosity of giant vesicle membranes prepared by spontaneous swelling, electroformation, gel-assisted, and phase transfer …

HA Faizi, A Tsui, R Dimova, PM Vlahovska - Langmuir, 2022 - ACS Publications
Closed lipid bilayers in the form of giant unilamellar vesicles (GUVs) are commonly used
membrane models. Various methods have been developed to prepare GUVs, however it is …

Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules

Y Elani, T Trantidou, D Wylie, L Dekker, K Polizzi… - Scientific reports, 2018 - nature.com
There is increasing interest in constructing artificial cells by functionalising lipid vesicles with
biological and synthetic machinery. Due to their reduced complexity and lack of evolved …