Evaluation of isolation methods and RNA integrity for bacterial RNA quantitation

CE Jahn, AO Charkowski, DK Willis - Journal of microbiological methods, 2008 - Elsevier
RNA integrity is critical for successful RNA quantitation for mammalian tissues, but the level
of integrity required differs among tissues. The level of integrity required for quantitation has …

The formation of the rodlet layer of streptomycetes is the result of the interplay between rodlins and chaplins

D Claessen, I Stokroos, HJ Deelstra… - Molecular …, 2004 - Wiley Online Library
Streptomycetes form hydrophobic aerial hyphae that eventually septate into hydrophobic
spores. Both aerial hyphae and spores possess a typical surface layer called the rodlet …

Antibiotic overproduction in Streptomyces coelicolor A3 (2) mediated by phosphofructokinase deletion

I Borodina, J Siebring, J Zhang, CP Smith… - Journal of Biological …, 2008 - ASBMB
Streptomycetes are exploited for production of a wide range of secondary metabolites, and
there is much interest in enhancing the level of production of these metabolites. Secondary …

Improved method for the isolation of RNA from bacteria refractory to disruption, including S. aureus producing biofilm

SS Atshan, MN Shamsudin, LTT Lung, KH Ling… - Gene, 2012 - Elsevier
The development of fast, reliable and inexpensive phenol protocol is described for the
isolation of RNA from bacterial biofilm producers. The method was tested on Staphylococcus …

An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads

CSY Lim, CH Tung, R Rosli, PP Chong - Journal of microbiological methods, 2008 - Elsevier
This report describes a modified, cost-effective method of cell wall disruption for the yeast
Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This …

An Improved RNA Isolation Method for Filamentous Fungus Blakeslea trispora Rich in Polysaccharides

Y Li, W Wang, X Du, Q Yuan - Applied biochemistry and biotechnology, 2010 - Springer
Isolation and purification of biologically active RNA from filamentous fungi is difficult
because of the complex cell wall and the high level of polysaccharides which bind to or co …

Protein secretion in Streptomyces

T Palmer, MI Hutchings - Streptomyces: Molecular Biology and …, 2011 - hero.epa.gov
The saprophytic lifestyle of Streptomyces requires them to secrete prolific numbers of
proteins. For example, inspection of the genome sequence of Streptomyces coelicolor …

[PDF][PDF] EVALUATION OF TOTAL RNA EXTRACTION METHODS FROM STAPHYLOCOCCUS AUREUS

SH Abdel-Rhman - J. Microbiol, 2016 - academia.edu
The analysis of RNA provides an excellent sign of gene expression profile of an organism.
The improvement of fast, easy and reliable glass beads/Trizol method is described for the …

[PDF][PDF] kk RNAMini, Trizoland MasterPure RNA extraction Kits using standard protocols consistently gavelow RNA yields

SS Atshan, MN Shamsudin, LTT Lung, KH Ling… - Gene, 2012 - faculty.uobasrah.edu.iq
RNA from 16S and 23S rRNA, contained 5S RNA. Higher yields due to improved biofilm
bacterial cell lysis were RT-qPCR achieved with an added hotphenolincubation step without …

Estructura poblacional de bacterias sulfato reductoras y arqueas metanogenas en perfiles sedimentarios de lagunas de aguas blancas y aguas negras de la cuenca …

K Salazar Chavez - 2009 - sidalc.net
La cuenca amazónica por su riqueza en materia orgánica y por las concentraciones
acumuladas de mercurio representa una zona de interés para el estudio de la diversidad …