Enlightening the path to protein engineering: chemoselective turn-on probes for high-throughput screening of enzymatic activity

S Hecko, A Schiefer, CPS Badenhorst, MJ Fink… - Chemical …, 2023 - ACS Publications
Many successful stories in enzyme engineering are based on the creation of randomized
diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods …

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

R Billot, L Plener, P Jacquet, M Elias… - Journal of Biological …, 2020 - ASBMB
Enzymes able to degrade or modify acyl-homoserine lactones (AHLs) have drawn
considerable interest for their ability to interfere with the bacterial communication process …

Engineering quorum quenching enzymes: progress and perspectives

SA Murugayah, ML Gerth - Biochemical Society Transactions, 2019 - portlandpress.com
Quorum sensing is a key contributor to the virulence of many important plant, animal and
human pathogens. The disruption of this signalling—a process referred to as 'quorum …

Two legume fatty acid amide hydrolase isoforms with distinct preferences for microbial-and plant-derived acylamides

O Arias-Gaguancela, E Herrell, M Aziz… - Scientific Reports, 2023 - nature.com
Fatty acid amide hydrolase (FAAH) is a widely conserved amidase in eukaryotes, perhaps
best known for inactivating N-acylethanolamine lipid mediators. However, FAAH enzymes …

Fatty acid amide hydrolase and 9-lipoxygenase modulate cotton seedling growth by ethanolamide oxylipin levels

O Arias-Gaguancela, M Aziz, KD Chapman - Plant Physiology, 2023 - academic.oup.com
Polyunsaturated N-acylethanolamines (NAEs) can be hydrolyzed by fatty acid amide
hydrolase (FAAH) or oxidized by lipoxygenase (LOX). In Arabidopsis (Arabidopsis thaliana) …

A review on the use of fluorescamine as versatile and convenient analytical probe

SM Derayea, E Samir - Microchemical Journal, 2020 - Elsevier
Fluorescamine (FSA) has been widely applied for the determination of several classes of
compounds containing amino groups. The estimated substances included pharmaceutical …

In Situ Preparation of Star-Shaped Protein–“Smart” Polymer Conjugates with pH and Thermo-Dual Responsibility for Bacterial Separation

F Wei, H Zheng, C Gao, J Tian, J Gou… - Journal of Agricultural …, 2024 - ACS Publications
To achieve effective separation and enrichment of bacteria, a novel synthetic scheme was
developed to synthesize star-style boronate-functionalized copolymers with excellent …

Exploring the potential of simple automation concepts for quantifying functional groups on nanomaterials with optical assays

I Tavernaro, A Matiushkina, KS Rother, C Mating… - Nano Research, 2024 - Springer
Until now, automation in nanomaterial research has been largely focused on the automated
synthesis of engineered nanoparticles (NPs) including the screening of synthesis …

Engineering quorum quenching acylases with improved kinetic and biochemical properties

K Sompiyachoke, MH Elias - Protein Science, 2024 - Wiley Online Library
Many Gram‐negative bacteria use N‐acyl‐L‐homoserine lactone (AHL) signals to
coordinate phenotypes such as biofilm formation and virulence factor production. Quorum …

[PDF][PDF] Влияние антибиотиков на систему «QUORUM SENSING» LUXI/LUXR-ТИПА у бактерий (на примере Chromobacterium violaceum)

КС Инчагова - 2020 - iegm.ru
Актуальность темы. Обнаружение плотностно-зависимой химической коммуникации у
бактерий, обозначенной термином «quorum sensing»(QS), стало одним из наиболее …