Structure, assembly, and function of tripartite efflux and type 1 secretion systems in gram-negative bacteria

I Alav, J Kobylka, MS Kuth, KM Pos, M Picard… - Chemical …, 2021 - ACS Publications
Tripartite efflux pumps and the related type 1 secretion systems (T1SSs) in Gram-negative
organisms are diverse in function, energization, and structural organization. They form …

Exploring cellular biochemistry with nanobodies

RW Cheloha, TJ Harmand, C Wijne… - Journal of Biological …, 2020 - ASBMB
Reagents that bind tightly and specifically to biomolecules of interest remain essential in the
exploration of biology and in their ultimate application to medicine. Besides ligands for …

Structural basis for dual-mode inhibition of the ABC transporter MsbA

H Ho, A Miu, MK Alexander, NK Garcia, A Oh… - Nature, 2018 - nature.com
The movement of core-lipopolysaccharide across the inner membrane of Gram-negative
bacteria is catalysed by an essential ATP-binding cassette transporter, MsbA. Recent …

Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor

S Gulati, H Jin, I Masuho, T Orban, Y Cai… - Nature …, 2018 - nature.com
G protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by mediating a GDP
to GTP exchange in the Gα subunit. This leads to dissociation of the heterotrimer into Gα …

Antigen recognition by single-domain antibodies: structural latitudes and constraints

KA Henry, CR MacKenzie - MAbs, 2018 - Taylor & Francis
ABSTRACT Single-domain antibodies (sdAbs), the autonomous variable domains of heavy
chain-only antibodies produced naturally by camelid ungulates and cartilaginous fishes …

Mechanics and pharmacology of substrate selection and transport by eukaryotic ABC exporters

S Srikant, R Gaudet - Nature structural & molecular biology, 2019 - nature.com
Much structural information has been amassed on ATP-binding cassette (ABC) transporters,
including hundreds of structures of isolated domains and an increasing array of full-length …

Insights into membrane curvature sensing and membrane remodeling by intrinsically disordered proteins and protein regions

C Has, P Sivadas, SL Das - The Journal of Membrane Biology, 2022 - Springer
Cellular membranes are highly dynamic in shape. They can rapidly and precisely regulate
their shape to perform various cellular functions. The protein's ability to sense membrane …

The extracellular gate shapes the energy profile of an ABC exporter

CAJ Hutter, MH Timachi, LM Hürlimann… - Nature …, 2019 - nature.com
ABC exporters harness the energy of ATP to pump substrates across membranes.
Extracellular gate opening and closure are key steps of the transport cycle, but the …

Structural and mechanistic studies of the N-glycosylation machinery: from lipid-linked oligosaccharide biosynthesis to glycan transfer

AS Ramírez, KP Locher - Glycobiology, 2023 - academic.oup.com
N-linked protein glycosylation is a post-translational modification that exists in all domains of
life. It involves two consecutive steps:(i) biosynthesis of a lipid-linked oligosaccharide (LLO) …

[HTML][HTML] Structural Basis of the Allosteric Inhibition of Human ABCG2 by Nanobodies

RN Irobalieva, I Manolaridis, SM Jackson, D Ni… - Journal of Molecular …, 2023 - Elsevier
ABCG2 is an ATP-binding cassette transporter that exports a wide range of xenobiotic
compounds and has been recognized as a contributing factor for multidrug resistance in …