The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence

J Kopeika, A Thornhill, Y Khalaf - Human reproduction update, 2015 - academic.oup.com
BACKGROUND Cryopreservation has been extensively used in assisted reproductive
technology, agriculture and conservation programmes for endangered species. The …

Linking genotoxic responses in Gammarus fossarum germ cells with reproduction impairment, using the Comet assay

E Lacaze, O Geffard, D Goyet, S Bony, A Devaux - Environmental research, 2011 - Elsevier
Germ cells perform a unique and critical biological function: they pass down DNA that will be
used for the development of the next generation. Thus there is an increasing need to …

Effect of type and concentration of cryoprotectant on the motility, viability, and fertility of climbing perch Anabas testudineus Bloch, 1792 (Pisces: Anabantidae) sperm

S Maulida, K Eriani, N Fadli, FK Kocabaş… - Theriogenology, 2023 - Elsevier
The climbing perch, Anabas testudineus is a freshwater fish that has economic value in
Indonesia. It is cultured in the country, but the breeding technology, specifically sperm …

Optimization of sperm cryopreservation protocol for Mediterranean brown trout: a comparative study of non-permeating cryoprotectants and thawing rates in vitro and …

G Rusco, M Di Iorio, PP Gibertoni, S Esposito… - Animals, 2019 - mdpi.com
Simple Summary Cryobanking is an important tool to preserve the genetic resources of fish
species. Semen cryopreservation has been extensively used in conservation programs for …

Cryoprotectant‐free ultra‐rapid freezing of human spermatozoa in cryogenic vials

J Zhu, RT Jin, LM Wu, L Johansson, TH Guo… - Andrologia, 2014 - Wiley Online Library
The objective of this study was to investigate the effect of ultra‐rapid freezing (direct
immersion in liquid nitrogen) on human spermatozoa in cryogenic vials (≥ 0.5 ml) at …

[PDF][PDF] 软鳍新光唇鱼精子的超低温冷冻保存

王晓爱, 杨君兴, 陈小勇, 潘晓赋, 李再云 - 动物学研究, 2012 - researchgate.net
2011 年, 对软鳍新光唇鱼(Neolissochilus benasi) 进行了精子超低温冷冻保存研究.
以解冻后的精子活力为指标, 采用稀释液D-15, 设计不同的抗冻剂种类和浓度 …

The effect of ions and cryoprotectants upon sperm motility and fertilization success in the loach Misgurnus anguillicaudatus

GS Yasui, T Fujimoto, L Arias-Rodriguez, Y Takagi… - Aquaculture, 2012 - Elsevier
The solutions commonly used to dilute or cryopreserve sperm are commonly composed of
salts, buffers and cryoprotectants, which may affect gametes and subsequent fertilization …

Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla

E Rodríguez-Pena, D Suárez, G Estévez-Pérez… - Animals, 2023 - mdpi.com
Simple Summary On one hand, the females of some crabs can store the sperm from
copulations for several years. On the other hand, it is reported that DNA repair mechanisms …

Use of methanol as cryoprotectant and its effect on sox genes and proteins in chilled zebrafish embryos

K Desai, E Spikings, T Zhang - Cryobiology, 2015 - Elsevier
Methanol is a widely used cryoprotectant (CPA) in cryopreservation of fish embryos,
however little is known about its effect at the molecular level. This study investigated the …

Effect of Different Extenders and Dilution Ratios on the Spermatological and Fertilization Parameters of Cyprinus carpio During Cryopreservation

CJ Betsy, JSS Kumar, KTP Jawahar - Biopreservation and …, 2019 - liebertpub.com
Production of quality seeds is a critical need, and cryopreservation serves as an important
biotechnological tool in achieving that goal. In the present study, the effects of different …