The burden of cardiovascular and metabolic diseases worldwide is staggering. The
emergence of systems approaches in biology promises new therapies, faster and cheaper …

Genome-editing tools, and in particular those based on CRISPR–Cas (clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein) systems, are …

A defining feature of HIV replication is integration of the proviral cDNA into human DNA. The
selection of chromosomal targets for integration is crucial for efficient viral replication, but the …

Heart disease is the leading cause of death worldwide. Despite decades of research, most
heart pathologies have limited treatments, and often the only curative approach is heart …

In Drosophila, enhancer trap strategies allow rapid access to expression patterns, molecular
data, and mutations in trapped genes. However, they do not give any information at the …

Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …

We report here development of a novel gene trap method in zebrafish using the Tol2
transposon system. First, we established a highly efficient transgenesis method in which a …

Genome sequencing has yielded a plethora of new genes the function of which can be
unravelled through comparative genomic approaches. Increasingly, developmental …

Identification and manipulation of the germ line are important to the study of model
organisms. Although zebrafish has recently emerged as a model for vertebrate …

A fundamental goal of genetics and functional genomics is to identify and mutate every gene
in model organisms such as Drosophila melanogaster. The Berkeley Drosophila Genome …