Microfluidics: reframing biological enquiry

TA Duncombe, AM Tentori, AE Herr - Nature Reviews Molecular Cell …, 2015 - nature.com
The underlying physical properties of microfluidic tools have led to new biological insights
through the development of microsystems that can manipulate, mimic and measure biology …

Recent developments in multiplexing techniques for immunohistochemistry

AR Dixon, C Bathany, M Tsuei, J White… - Expert review of …, 2015 - Taylor & Francis
Methods to detect immunolabeled molecules at increasingly higher resolutions, even when
present at low levels, are revolutionizing immunohistochemistry (IHC). These technologies …

Proteomics in cancer biomarkers discovery: challenges and applications

RM Sallam - Disease markers, 2015 - Wiley Online Library
With the introduction of recent high‐throughput technologies to various fields of science and
medicine, it is becoming clear that obtaining large amounts of data is no longer a problem in …

Preclinical models and technologies to advance nanovaccine development

C Peres, AI Matos, LIF Moura, RC Acurcio… - Advanced Drug Delivery …, 2021 - Elsevier
The remarkable success of targeted immunotherapies is revolutionizing cancer treatment.
However, tumor heterogeneity and low immunogenicity, in addition to several tumor …

Dual signal amplification by polysaccharide and eATRP for ultrasensitive detection of CYFRA 21–1 DNA

L Zhao, H Yang, X Zheng, J Li, L Jian, W Feng… - Biosensors and …, 2020 - Elsevier
Abstract Cytokeratin fragment antigen 21–1 (CYFRA 21–1) DNA is a crucial biomarker
closely associated with non-small cell lung cancer. Here, we fabricated a novel …

Exploring living multicellular organisms, organs, and tissues using microfluidic systems

V Sivagnanam, MAM Gijs - Chemical reviews, 2013 - ACS Publications
Lab-on-a-chip (LoC) systems allow the implementation of benchtop analytical
methods/protocols using low reagent volumes, short processing times, and this potentially at …

Biomarkers in abnormal uterine bleeding

R Chodankar, HOD Critchley - Biology of reproduction, 2019 - academic.oup.com
Abnormal uterine bleeding (AUB) is an extremely common problem and represents a clinical
area of unmet need. It has clinical implications and a high cost for the healthcare system …

Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs

AT Ciftlik, HA Lehr, MAM Gijs - Proceedings of the National …, 2013 - National Acad Sciences
Biomarker analysis is playing an essential role in cancer diagnosis, prognosis, and
prediction. Quantitative assessment of immunohistochemical biomarker expression on tumor …

Efficient AC electrothermal flow (ACET) on-chip for enhanced immunoassays

MS Draz, K Uning, D Dupouy, MAM Gijs - Lab on a Chip, 2023 - pubs.rsc.org
Biochemical reaction rates in microfluidic systems are known to be limited by the diffusional
transport of reagents, leading often to lowered sensitivity and/or longer detection times in …

193 nm ultraviolet photodissociation mass spectrometry for phosphopeptide characterization in the positive and negative ion modes

MR Robinson, JM Taliaferro, KN Dalby… - Journal of proteome …, 2016 - ACS Publications
Advances in liquid chromatography tandem mass spectrometry (LC–MS/MS) have permitted
phosphoproteomic analysis on a grand scale, but ongoing challenges specifically …