Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Chemical approaches to optimize the properties of organic fluorophores for imaging and sensing

G Jiang, H Liu, H Liu, G Ke, TB Ren… - Angewandte …, 2024 - Wiley Online Library
Organic fluorophores are indispensable tools in cells, tissue and in vivo imaging, and have
enabled much progress in the wide range of biological and biomedical fields. However …

Near‐Infrared Self‐Assembled Hydroxyl Radical Generator Based on Photoinduced Cascade Electron Transfer for Hypoxic Tumor Phototherapy

X Zhao, S He, J Wang, J Ding, S Zong, G Li… - Advanced …, 2023 - Wiley Online Library
The hydroxyl radical (• OH) is an extremely potent reactive oxygen species that plays a
crucial role in photooxidations within the realm of hypoxic tumor therapy. However, the …

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

DA Helmerich, G Beliu, D Taban, M Meub, M Streit… - Nature …, 2022 - nature.com
Advances in super-resolution microscopy have demonstrated single-molecule localization
precisions of a few nanometers. However, translation of such high localization precisions …

The design and bioimaging applications of NIR fluorescent organic dyes with high brightness

J Wu, Z Shi, L Zhu, J Li, X Han, M Xu… - Advanced Optical …, 2022 - Wiley Online Library
Due to the merits of decreased photon attenuation, autofluorescence, and scattering, the
near‐infrared (NIR, 700–1700 nm) region is an important window in the field of biomedicine …

Photochemical mechanisms of fluorophores employed in single‐molecule localization microscopy

K Kikuchi, LD Adair, J Lin, EJ New… - Angewandte Chemie, 2023 - Wiley Online Library
Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

E Lerner, A Barth, J Hendrix, B Ambrose, V Birkedal… - Elife, 2021 - elifesciences.org
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Visible‐Light‐Driven Rapid 3D Printing of Photoresponsive Resins for Optically Clear Multifunctional 3D Objects

S Shin, Y Kwon, C Hwang, W Jeon, Y Yu… - Advanced …, 2024 - Wiley Online Library
Light‐driven 3D printing is gaining significant attention for its unparalleled build speed and
high‐resolution in additive manufacturing. However, extending vat photopolymerization to …

Mechanism of cyanine5 to cyanine3 photoconversion and its application for high-density single-particle tracking in a living cell

Y Cho, HJ An, T Kim, C Lee, NK Lee - Journal of the American …, 2021 - ACS Publications
Cyanine (Cy) dyes are among the most useful organic fluorophores that have found a wide
range of applications in single-molecule and super-resolution imaging as well as in other …

A general strategy to develop fluorogenic polymethine dyes for bioimaging

A Martin, P Rivera-Fuentes - Nature Chemistry, 2024 - nature.com
Fluorescence imaging is an invaluable tool to study biological processes and further
progress depends on the development of advanced fluorogenic probes that reach …