Uncovering the cis-regulatory code that governs when and how much each gene is
transcribed in a given genome and cellular state remains a central goal of biology. Here, we …

Enhancers are central for the regulation of metazoan transcription but have proven difficult to
study, primarily due to a myriad of interdependent variables shaping their activity …

Transcriptional effectors are protein domains known to activate or repress gene expression;
however, a systematic understanding of which effector domains regulate transcription across …

Histone H3. 3 is frequently mutated in tumors, with the lysine 36 to methionine mutation
(K36M) being a hallmark of chondroblastomas. While it is known that H3. 3K36M changes …

Epigenetic regulation involves the coordinated interplay of diverse proteins. To
systematically explore these combinations, we present COMBINE (combinatorial interaction …

Multiplexed genetic perturbations are critical for testing functional interactions among coding
or non-coding genetic elements. Compared to double-stranded DNA cutting, repressive …

The construction of synthetic gene circuits requires the rational combination of multiple
regulatory components, but predicting their behavior can be challenging due to poorly …

Targeted epigenome editing tools allow precise manipulation and investigation of genome
modifications, however they often display high context dependency and variable efficacy …

Summary Most human Transcription factors (TFs) genes encode multiple protein isoforms
differing in DNA binding domains, effector domains, or other protein regions. The global …

The spatiotemporal control of gene expression in complex multicellular organisms relies on
noncoding regulatory sequences such as enhancers, which activate transcription of target …