Cryo‐electron microscopy of vitreous sections

A Al‐Amoudi, JJ Chang, A Leforestier… - The EMBO …, 2004 - embopress.org
Since the beginning of the 1980s, cryo‐electron microscopy of a thin film of vitrified aqueous
suspension has made it possible to observe biological particles in their native state, in the …

Amorphous water

CA Angell - Annu. Rev. Phys. Chem., 2004 - annualreviews.org
▪ Abstract After providing some background material to establish the interest content of this
subject, we summarize the many different ways in which water can be prepared in the …

Cryo-Transmission Electron Microscopy of Frozen-Hydrated Sections of Escherichia coli and Pseudomonas aeruginosa

VRF Matias, A Al-Amoudi, J Dubochet… - Journal of …, 2003 - Am Soc Microbiol
High-pressure freezing of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1 in the
presence of cryoprotectants provided consistent vitrification of cells so that frozen-hydrated …

Cryo‐electron microscopy reveals native polymeric cell wall structure in Bacillus subtilis 168 and the existence of a periplasmic space

VRF Matias, TJ Beveridge - Molecular microbiology, 2005 - Wiley Online Library
Ultrarapid freezing of bacteria (ie vitrification) results in optimal preservation of native
structure. In this study, cryo‐transmission electron microscopy of frozen‐hydrated sections …

Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy

A Al-Amoudi, D Studer, J Dubochet - Journal of structural biology, 2005 - Elsevier
Cryo-electron microscopy of vitreous sections (CEMOVIS) has recently been shown to
provide images of biological specimens with unprecedented quality and resolution. Cutting …

Cryo-electron microscopy of vitreous sections of native biological cells and tissues

A Al-Amoudi, LPO Norlen, J Dubochet - Journal of structural biology, 2004 - Elsevier
Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate
method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of …

Amorphous ices: experiments and numerical simulations

T Loerting, N Giovambattista - Journal of physics: Condensed …, 2006 - iopscience.iop.org
Polyamorphism, ie the presence of more than one amorphous state, was observed for the
first time in amorphous ice or glassy water. In addition to LDA (low-density amorphous ice), a …

Frozen piercing implements and methods for piercing a substrate

ES Boyden, DB Cook, RA Hyde, EC Leuthardt… - US Patent …, 2014 - Google Patents
2009-12-03 Assigned to SEARETE LLC reassignment SEARETE LLC ASSIGNMENT OF
ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: COOK, DANIEL B …

Electron microscopy of pharmaceutical systems

V Klang, C Valenta, NB Matsko - Micron, 2013 - Elsevier
During the last decades, the focus of research in pharmaceutical technology has steadily
shifted towards the development and optimisation of nano-scale drug delivery systems. As a …

Structure determination by single-particle cryo-electron microscopy: only the sky (and intrinsic disorder) is the limit

E Nwanochie, VN Uversky - International journal of molecular sciences, 2019 - mdpi.com
Traditionally, X-ray crystallography and NMR spectroscopy represent major workhorses of
structural biologists, with the lion share of protein structures reported in protein data bank …