Nuclear DNA amounts in angiosperms: targets, trends and tomorrow

MD Bennett, IJ Leitch - Annals of botany, 2011 - academic.oup.com
Abstract Background and Aims The amount of DNA in an unreplicated gametic chromosome
complement is known as the C-value and is a key biodiversity character of fundamental …

Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications

AK Neelakandan, K Wang - Plant cell reports, 2012 - Springer
In vitro cell and tissue-based systems have tremendous potential in fundamental research
and for commercial applications such as clonal propagation, genetic engineering and …

Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species

J Loureiro, E Rodriguez, J Doležel, C Santos - Annals of botany, 2007 - academic.oup.com
Abstract Background and Aims After the initial boom in the application of flow cytometry in
plant sciences in the late 1980s and early 1990s, which was accompanied by development …

Somaclonal variation in tissue culture: a case study with olive

AR Leva, R Petruccelli… - Recent advances in plant …, 2012 - books.google.com
Micropropagation of woody plants and fruit crops constitutes a major success in the
commercial application of in vitro cultures. An important aspect to be considered when …

Unlocking grapevine in vitro regeneration: Issues and perspectives for genetic improvement and functional genomic studies

F Nuzzo, G Gambino, I Perrone - Plant Physiology and Biochemistry, 2022 - Elsevier
In vitro plant regeneration is a pivotal process in genetic engineering to obtain large
numbers of transgenic, cisgenic and gene edited plants in the frame of functional gene or …

High Genetic and Epigenetic Stability in Coffea arabica Plants Derived from Embryogenic Suspensions and Secondary Embryogenesis as Revealed by AFLP, MSAP …

R Bobadilla Landey, A Cenci, F Georget, B Bertrand… - PLOS …, 2013 - journals.plos.org
Embryogenic suspensions that involve extensive cell division are risky in respect to genome
and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic …

Flow cytometry–a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

E Sliwinska - Folia Horticulturae, 2018 - sciendo.com
Flow cytometry (FCM) has been used for plant DNA content estimation since the 1980s;
however, presently, the number of laboratories equipped with flow cytometers has …

CRISPR/Cas9 editing of Downy mildew resistant 6 (DMR6-1) in grapevine leads to reduced susceptibility to Plasmopara viticola

S Djennane, S Gersch, F Le-Bohec… - Journal of …, 2024 - academic.oup.com
Downy mildew of grapevine (Vitis vinifera), caused by the oomycete Plasmopara viticola, is
an important disease that is present in cultivation areas worldwide, and using resistant …

Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

MJ Prado, E Rodriguez, L Rey, MV González… - Plant Cell, Tissue and …, 2010 - Springer
Flow cytometry and microsatellite analyses were used to evaluate the trueness-to-type of
somatic embryogenesis-regenerated plants from six important Spanish grapevine (Vitis …

Autotetraploid Emergence via Somatic Embryogenesis in Vitis vinifera Induces Marked Morphological Changes in Shoots, Mature Leaves, and Stomata

C Catalano, L Abbate, A Motisi, D Crucitti, V Cangelosi… - Cells, 2021 - mdpi.com
Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses.
Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) …