Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Tumor cells do not exist in isolation in vivo, and carcinogenesis depends on the surrounding
tumor microenvironment (TME), composed of a myriad of cell types and biophysical and …

Molecular characterization of cell types using single-cell transcriptome sequencing is
revolutionizing cell biology and enabling new insights into the physiology of human organs …

INTRODUCTION Cancer immunotherapies that target tumor-specific T cells have benefited
many cancer patients, but the clinical efficacy varies greatly among different cancer types …

Cellular function in tissue is dependent on the local environment, requiring new methods for
spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial …

A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues
into single-cell suspensions. While many protocols have been optimized for optimal cell …

Tumor microenvironment (TME) is a specialized ecosystem of host components, designed
by tumor cells for successful development and metastasis of tumor. With the advent of 3D …

Single-cell RNA sequencing (scRNA-seq) identifies cell subpopulations within tissue but
does not capture their spatial distribution nor reveal local networks of intercellular …

Fibroblasts are non-haematopoietic structural cells that define the architecture of organs,
support the homeostasis of tissue-resident cells and have key roles in fibrosis, cancer …

Abstract PD-1 blockade unleashes CD8 T cells, including those specific for mutation-
associated neoantigens (MANA), but factors in the tumour microenvironment can inhibit …