Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

DR Yáñez-Ruiz, A Bannink, J Dijkstra… - Animal Feed Science …, 2016 - Elsevier
In vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value
of feeds for ruminants and in the last decade to assess the effect of different nutritional …

In vitro microbial inoculum: A review of its function and properties

FL Mould, KE Kliem, R Morgan, RM Mauricio - Animal Feed Science and …, 2005 - Elsevier
This review considers microbial inocula used in in vitro systems from the perspective of their
ability to degrade or ferment a particular substrate, rather than the microbial species that it …

In vitro cumulative gas production techniques: History, methodological considerations and challenges

C Rymer, JA Huntington, BA Williams… - Animal Feed Science and …, 2005 - Elsevier
Methodology used to measure in vitro gas production is reviewed to determine impacts of
sources of variation on resultant gas production profiles (GPP). Current methods include …

Influence of inoculum source in a gas production method

ICS Bueno, SLS Cabral Filho, SP Gobbo… - Animal feed science and …, 2005 - Elsevier
Gas production techniques are used in many laboratories to study fermentation kinetics of
ruminant feeds, and the major source of variation is often the inoculum. Fifteen substrates …

Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques

G Hervás, P Frutos, FJ Giráldez, MJ Mora… - Animal Feed Science …, 2005 - Elsevier
Use of preserved rumen fluid (RF) as microbial inoculum for in vitro gas production
techniques (IVGPT) would be of practical importance when access to ruminally cannulated …

The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters

S Nagadi, M Herrero, NS Jessop - Animal Feed Science and Technology, 2000 - Elsevier
Six sheep were fed twice a day on a different ratio of sheep pellets and hay (20: 80 (diet 1),
40: 60 (diet 2) and 80: 20 (diet 3)) in a replicated Latin square design to study the effect of …

[HTML][HTML] An alternative method to assess 24-h ruminal in vitro neutral detergent fiber digestibility

JP Goeser, DK Combs - Journal of Dairy Science, 2009 - Elsevier
Interassay error caused by the inconsistent nature of rumen fluid inoculum confounds
comparisons of forage in vitro neutral detergent fiber (NDF) digestibility (NDFD) analyzed in …

In vitro fermentation characteristics of diets with different forage/concentrate ratios: comparison of rumen and faecal inocula

F Zicarelli, S Calabro, MI Cutrignelli… - Journal of the …, 2011 - Wiley Online Library
BACKGROUND: The aim of this trial was to evaluate the replacement of rumen fluid with
faeces as inoculum in studying the in vitro fermentation characteristics of diets for ruminants …

Modulation of Rumen Microbes Through Extracellular Vesicle Released by the Rumen Fluke Calicophoron daubneyi

NR Allen, AR Taylor-Mew, TJ Wilkinson… - Frontiers in Cellular …, 2021 - frontiersin.org
Parasite derived extracellular vesicles (EVs) have been proposed to play key roles in the
establishment and maintenance of infection. Calicophoron daubneyi is a newly emerging …

A new in vitro system to study the effect of liquid phase turnover and pH on microbial fermentation of concentrate diets for ruminants

M Fondevila, B Pérez-Espés - Animal Feed Science and Technology, 2008 - Elsevier
A simple and inexpensive in vitro feed evaluation system which estimates microbial
fermentation by measuring gas production and allows for adjustment of liquid dilution rate …