Proximity dependent biotinylation: key enzymes and adaptation to proteomics approaches

P Samavarchi-Tehrani, R Samson… - Molecular & Cellular …, 2020 - ASBMB
The study of protein subcellular distribution, their assembly into complexes and the set of
proteins with which they interact with is essential to our understanding of fundamental …

Short open reading frame genes in innate immunity: from discovery to characterization

E Malekos, S Carpenter - Trends in immunology, 2022 - cell.com
Next-generation sequencing (NGS) technologies have greatly expanded the size of the
known transcriptome. Many newly discovered transcripts are classified as long noncoding …

[HTML][HTML] Engineering AraC to make it responsive to light instead of arabinose

E Romano, A Baumschlager, EB Akmeric… - Nature chemical …, 2021 - nature.com
The l-arabinose-responsive AraC and its cognate PBAD promoter underlie one of the most
often used chemically inducible prokaryotic gene expression systems in microbiology and …

The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants

L Chen, GB Jameson, Y Guo, J Song… - Plant Biotechnology …, 2022 - Wiley Online Library
Summary LONELY GUY (LOG) was first identified in a screen of rice mutants with defects in
meristem maintenance. In plants, LOG codes for cytokinin riboside 5′‐monophosphate …

Knockout of the sugar transporter OsSTP15 enhances grain yield by improving tiller number due to increased sugar content in the shoot base of rice (Oryza sativa L.)

M Li, H Li, Q Zhu, D Liu, Z Li, H Chen, J Luo… - New …, 2024 - Wiley Online Library
Sugar transporter proteins (STPs) play critical roles in regulating plant stress tolerance,
growth, and development. However, the role of STPs in regulating crop yield is poorly …

Proximity-dependent biotinylation approaches to explore the dynamic compartmentalized proteome

U Dionne, AC Gingras - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and
their derivatives, have been widely used to define the compositions of organelles and other …

Membrane protein stabilization strategies for structural and functional studies

E Errasti-Murugarren, P Bartoccioni, M Palacín - Membranes, 2021 - mdpi.com
Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly
relevant for cell physiology and pharmacology. In this regard, the structural determination of …

Genome-wide conditional degron libraries for functional genomics

E Gameiro, KA Juárez-Núñez, JJ Fung… - Journal of Cell …, 2025 - rupress.org
Functional genomics with libraries of knockout alleles is limited to non-essential genes and
convoluted by the potential accumulation of suppressor mutations in knockout backgrounds …

Proximity‐dependent biotinylation approaches to study apicomplexan biology

J Kimmel, J Kehrer, F Frischknecht… - Molecular …, 2022 - Wiley Online Library
In the last 10 years, proximity‐dependent biotinylation (PDB) techniques greatly expanded
the ability to study protein environments in the living cell that range from specific protein …

Identification of structural motifs critical for human G6PC2 function informed by sequence analysis and an AlphaFold2-predicted model

EM Hawes, DP Claxton, JK Oeser… - Bioscience …, 2024 - portlandpress.com
G6PC2 encodes a glucose-6-phosphatase (G6Pase) catalytic subunit, primarily expressed
in pancreatic islet β cells, which modulates the sensitivity of insulin secretion to glucose and …