Cell-based scaffold-free therapies seek to develop in vitro organotypic three-dimensional
(3D) tissue-like surrogates, capitalising upon the inherent capacity of cells to create tissues …

The intracellular environment represents an extremely crowded milieu, with a limited amount
of free water and an almost complete lack of unoccupied space. Obviously, slightly salted …

Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays.
They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic …

Complex coacervation driven liquid-liquid phase separation (LLPS) of biopolymers has
been attracting attention as a novel phase in living cells. Studies of LLPS in this context are …

Intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs)
are functional proteins and domains that devoid stable secondary and/or tertiary structure …

The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain
a detailed understanding of GSH‐related pathological events. However, owing to their …

Efficient folding of many newly synthesized proteins depends on assistance from molecular
chaperones, which serve to prevent protein misfolding and aggregation in the crowded …

Biological macromolecules evolve and function within intracellular environments that are
crowded with other macromolecules. Crowding results in surprisingly large quantitative …

Biological macromolecules have evolved over billions of years to function inside cells, so it
is not surprising that researchers studying the properties of such molecules, either in extracts …

Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …