[HTML][HTML] Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: achievements and future direction

AKL Chen, S Reuveny, SKW Oh - Biotechnology advances, 2013 - Elsevier
Mesenchymal stem cells (MSCs) have recently made significant progress with multiple
clinical trials targeting modulation of immune responses, regeneration of bone, cartilage …

Chemically defined generation of human cardiomyocytes

PW Burridge, E Matsa, P Shukla, ZC Lin, JM Churko… - Nature …, 2014 - nature.com
Existing methods for human induced pluripotent stem cell (hiPSC) cardiac differentiation are
efficient but require complex, undefined medium constituents that hinder further elucidation …

Design of polymeric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing

A Higuchi, QD Ling, SS Kumar, M Munusamy… - Progress in Polymer …, 2014 - Elsevier
This review describes recent developments regarding the use of natural and synthetic
polymers to support the propagation of human pluripotent stem cells (hPSCs), human …

[HTML][HTML] Critical microcarrier properties affecting the expansion of undifferentiated human embryonic stem cells

AKL Chen, X Chen, ABH Choo, S Reuveny, SKW Oh - Stem cell research, 2011 - Elsevier
A variety of microcarriers may be used for the expansion of human embryonic stem cells
(hESC) for cell therapy applications. This study investigated the effects of 10 types of …

Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity

A Higuchi, SH Kao, QD Ling, YM Chen, HF Li… - Scientific reports, 2015 - nature.com
The tentative clinical application of human pluripotent stem cells (hPSCs), such as human
embryonic stem cells and human induced pluripotent stem cells, is restricted by the …

Facile engineering of xeno-free microcarriers for the scalable cultivation of human pluripotent stem cells in stirred suspension

Y Fan, M Hsiung, C Cheng… - Tissue Engineering Part …, 2014 - liebertpub.com
A prerequisite for the realization of human pluripotent stem cell (hPSC) therapies is the
development of bioprocesses for generating clinically relevant quantities of undifferentiated …

Translating human embryonic stem cells from 2-dimensional to 3-dimensional cultures in a defined medium on laminin-and vitronectin-coated surfaces

BC Heng, J Li, AKL Chen, S Reuveny… - Stem cells and …, 2012 - liebertpub.com
While defining the environment for human embryonic stem cell (hESC) culture on 2-
dimensional (2D) surfaces has made rapid progress, the industrial-scale implementation of …

[HTML][HTML] In vivo generation of post-infarct human cardiac muscle by laminin-promoted cardiovascular progenitors

L Yap, JW Wang, A Moreno-Moral, LY Chong, Y Sun… - Cell reports, 2019 - cell.com
Regeneration of injured human heart muscle is limited and an unmet clinical need. There
are no methods for the reproducible generation of clinical-quality stem cell-derived …

Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs

YM Chen, LH Chen, MP Li, HF Li, A Higuchi… - Scientific reports, 2017 - nature.com
Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in
xeno-free conditions is essential for producing clinical-grade cells. Development of cell …

The impact of adhesion molecules on the in vitro culture and differentiation of stem cells

A Abdal Dayem, S Lee, H Y. Choi… - Biotechnology …, 2018 - Wiley Online Library
The term “cell adhesion” represents cell–cell interactions and the interaction between the
cell and the extracellular matrix (ECM). These interactions are crucial for the development of …