POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells
POLQ is a key effector of DSB repair by microhomology-mediated end-joining (MMEJ) and is
overexpressed in many cancers. POLQ inhibitors confer synthetic lethality in HR and …
overexpressed in many cancers. POLQ inhibitors confer synthetic lethality in HR and …
[HTML][HTML] PARP1-DNA co-condensation drives DNA repair site assembly to prevent disjunction of broken DNA ends
DNA double-strand breaks (DSBs) are repaired at DSB sites. How DSB sites assemble and
how broken DNA is prevented from separating is not understood. Here we uncover that the …
how broken DNA is prevented from separating is not understood. Here we uncover that the …
Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein
Homologous recombination (HR) is essential for error-free repair of DNA double-strand
breaks, perturbed replication forks (RFs), and post-replicative single-stranded DNA (ssDNA) …
breaks, perturbed replication forks (RFs), and post-replicative single-stranded DNA (ssDNA) …
FANCD2–FANCI surveys DNA and recognizes double-to single-stranded junctions
DNA crosslinks block DNA replication and are repaired by the Fanconi anaemia pathway.
The FANCD2–FANCI (D2–I) protein complex is central to this process as it initiates repair by …
The FANCD2–FANCI (D2–I) protein complex is central to this process as it initiates repair by …
Insights into the control of RAD51 nucleoprotein filament dynamics from single-molecule studies
F Morati, M Modesti - Current Opinion in Genetics & Development, 2021 - Elsevier
Genomic integrity depends on the RecA/RAD51 protein family. Discovered over five
decades ago with the founder bacterial RecA protein, eukaryotic RAD51 is an ATP …
decades ago with the founder bacterial RecA protein, eukaryotic RAD51 is an ATP …
Search and processing of Holliday junctions within long DNA by junction-resolving enzymes
AP Kaczmarczyk, AC Déclais, MD Newton… - Nature …, 2022 - nature.com
Resolution of Holliday junctions is a critical intermediate step of homologous recombination
in which junctions are processed by junction-resolving endonucleases. Although binding …
in which junctions are processed by junction-resolving endonucleases. Although binding …
De novo fabrication of custom-sequence plasmids for the synthesis of long DNA constructs with extrahelical features
DNA constructs for single-molecule experiments often require specific sequences and/or
extrahelical/noncanonical structures to study DNA-processing mechanisms. The precise …
extrahelical/noncanonical structures to study DNA-processing mechanisms. The precise …
Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers
C Aicart-Ramos, S Hormeno, OJ Wilkinson… - Methods in …, 2022 - Elsevier
Single molecule biophysics experiments for the study of DNA-protein interactions usually
require production of a homogeneous population of long DNA molecules with controlled …
require production of a homogeneous population of long DNA molecules with controlled …
Visualizing the dynamics of DNA replication and repair at the single-molecule level
During cell division, the genome of each eukaryotic cell is copied by thousands of
replisomes—large protein complexes consisting of several dozen proteins. Recent studies …
replisomes—large protein complexes consisting of several dozen proteins. Recent studies …
[PDF][PDF] Mechanics and execution of homologous recombination: a single-molecule view
O Belan - 2022 - core.ac.uk
Homologous recombination (HR) is an essential mechanism for the repair of toxic DNA
double-strand breaks (DSBs), which, when not repaired accurately, can give rise to cancer …
double-strand breaks (DSBs), which, when not repaired accurately, can give rise to cancer …