Endemic plant species are usually more vulnerable to anthropogenic threats and natural
changes and, therefore, hold a higher extinction risk. The preservation of these species is a …

In the fifty years since the establishment of the cryoprotective effect of glycerol, cell banking
by cryopreservation has become routine in many areas of biotechnology and medicine …

In vitro plant cells, tissues and organ cultures are not fully autotrophic establishing a need for
carbohydrates in culture media to maintain the osmotic potential, as well as to serve as …

In vitro techniques have found increasing use in the conservation of threatened plants in
recent years and this trend is likely to continue as more species face risk of extinction. The …

Many plants cannot vitrify themselves because they lack glassy state-inducing substances
and/or have high water content. Therefore, cryoprotectants are used to induce vitrification. A …

The behaviour of water in liquid and frozen states is largely dictated by its bipolar chemistry.
Its hydrogen atoms carry a slight net positive charge and act with unpaired “negatively …

Plant cryopreservation involves the storage of plant tissues (usually seed or shoot tips) in
liquid nitrogen (LN) at-196 C or in the vapour phase of LN at-135 C in such a way that the …

This review addresses a frequently encountered problem of designing an effective
cryopreservation procedure for new (not previously cryopreserved) or difficult plant …

This study reports on the first use of a vitrification procedure for the successful
cryopreservation of embryogenic cultures of a coniferous species. Using Picea mariana …

A simple cryopreservation method is described for proliferating meristem cultures of banana
(Musa spp.). It relies on a 2-week preculture on media containing 0.4 M sucrose followed by …