Komagataella phaffii as Emerging Model Organism in Fundamental Research

L Bernauer, A Radkohl, LGK Lehmayer… - Frontiers in …, 2021 - frontiersin.org
Komagataella phaffii (Pichia pastoris) is one of the most extensively applied yeast species in
pharmaceutical and biotechnological industries, and, therefore, also called the biotech …

Towards systems metabolic engineering in Pichia pastoris

JP Schwarzhans, T Luttermann, M Geier… - Biotechnology …, 2017 - Elsevier
The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

P Cai, X Duan, X Wu, L Gao, M Ye… - Nucleic Acids …, 2021 - academic.oup.com
The industrial yeast Pichia pastoris has been harnessed extensively for production of
proteins, and it is attracting attention as a chassis cell factory for production of chemicals …

Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names

AP Douglass, B Offei, S Braun-Galleani… - PLoS …, 2018 - journals.plos.org
We investigated genomic diversity of a yeast species that is both an opportunistic pathogen
and an important industrial yeast. Under the name Candida krusei, it is responsible for about …

Genome diversity and evolution in the budding yeasts (Saccharomycotina)

BA Dujon, EJ Louis - Genetics, 2017 - academic.oup.com
Considerable progress in our understanding of yeast genomes and their evolution has been
made over the last decade with the sequencing, analysis, and comparisons of numerous …

Systems biotechnology for protein production in Pichia pastoris

RJ Zahrl, DA Peña, D Mattanovich… - FEMS yeast …, 2017 - academic.oup.com
The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

An evolutionary perspective on yeast mating-type switching

SJ Hanson, KH Wolfe - Genetics, 2017 - academic.oup.com
Cell differentiation in yeast species is controlled by a reversible, programmed DNA-
rearrangement process called mating-type switching. Switching is achieved by two …

Recent advances in domesticating non‐model microorganisms

Z Fatma, JC Schultz, H Zhao - Biotechnology progress, 2020 - Wiley Online Library
Non‐model microorganisms have been increasingly explored as microbial cell factories for
production of chemicals, fuels, and materials owing to their unique physiology and metabolic …

What makes Komagataella phaffii non-conventional?

Ö Ata, BG Ergün, P Fickers, L Heistinger… - FEMS Yeast …, 2021 - academic.oup.com
The important industrial protein production host Komagataella phaffii (syn Pichia pastoris) is
classified as a non-conventional yeast. But what exactly makes K. phaffii non-conventional …

Multiple reinventions of mating-type switching during budding yeast evolution

T Krassowski, J Kominek, XX Shen, DA Opulente… - Current Biology, 2019 - cell.com
Cell type in budding yeasts is determined by the genotype at the mating-type (MAT) locus,
but yeast species differ widely in their mating compatibility systems and life cycles. Among …