The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …

Ever since Caenorhabditis elegans was introduced as a model system it has been tightly
linked to microscopy, which has led to significant advances in understanding biology over …

Apical extracellular matrices (aECMs) are complex extracellular compartments that form
important interfaces between animals and their environment. In the adult C. elegans cuticle …

Mitochondria are organelles with their own genomes, but they rely on the import of nuclear-
encoded proteins that are translated by cytosolic ribosomes. Therefore, it is important to …

CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high
mutation efficiencies have been reported in primary transformants. However, many of the …

The lack of efficient tools to image non-repetitive genes in living cells has limited our ability
to explore the functional impact of the spatiotemporal dynamics of such genes. Here, we …

Cilia are sensory organelles protruding from cell surfaces. Release of extracellular vesicles
(EVs) from cilia was previously observed in mammals, Chlamydomonas, and in male …

Efficient and reproducible transgenesis facilitates and accelerates research using genetic
model organisms. Here, we describe a mo dular s afe-harbor t ransgene i nsertion (MosTI) …

During aging, preservation of locomotion is generally considered an indicator of sustained
good health, in elderlies and in animal models. In Caenorhabditis elegans, mutants of the …

The stable incorporation of transgenes and recombinant DNA material into the host genome
is a bottleneck in many bioengineering applications. Due to the low efficiency, identifying the …