RNA‐Seq methods for transcriptome analysis

R Hrdlickova, M Toloue, B Tian - Wiley Interdisciplinary …, 2017 - Wiley Online Library
Deep sequencing has been revolutionizing biology and medicine in recent years, providing
single base‐level precision for our understanding of nucleic acid sequences in high …

Dynamic post-transcriptional events governing CD8+ T cell homeostasis and effector function

F Salerno, M Turner, MC Wolkers - Trends in immunology, 2020 - cell.com
Effective T cell responses against infections and tumors require a swift and ample
production of cytokines, chemokines, and cytotoxic molecules. The production of these …

The RNA binding protein FgRbp1 regulates specific pre-mRNA splicing via interacting with U2AF23 in Fusarium

M Wang, T Ma, H Wang, J Liu, Y Chen… - Nature …, 2021 - nature.com
Precursor messenger RNA (pre-mRNA) splicing is an essential and tightly regulated
process in eukaryotic cells; however, the regulatory mechanisms for the splicing are not well …

Next-generation sequencing: A new avenue to understand viral RNA–protein interactions

Y Zhou, SL Sotcheff, AL Routh - Journal of Biological Chemistry, 2022 - ASBMB
The genomes of RNA viruses present an astonishing source of both sequence and structural
diversity. From intracellular viral RNA-host interfaces to interactions between the RNA …

Small-molecule Ro-08-2750 interacts with many RNA-binding proteins and elicits MUSASHI2-independent phenotypes

K Walters, MP Sajek, E Murphy, A Issaian, A Baldwin… - RNA, 2023 - rnajournal.cshlp.org
RNA-binding proteins (RBPs) are key regulators of gene expression. Small molecules
targeting these RBP–RNA interactions are a rapidly emerging class of therapeutics for …

Bacterial regulatory RNAs: complexity, function, and putative drug targeting

HL Cheah, CA Raabe, LP Lee… - Critical Reviews in …, 2018 - Taylor & Francis
Over the past decade, RNA-deep sequencing has uncovered copious non-protein coding
RNAs (npcRNAs) in bacteria. Many of them are key players in the regulation of gene …

Experimental methods to study protein–nucleic acid interactions

R Giambruno, J Rupert, E Zacco - Advances in Protein Molecular and …, 2022 - Elsevier
The interactions between proteins and nucleic acids regulate gene expression at
transcriptional, post-transcriptional, and translational levels and often dictate the fate of …

Investigation of RNA Editing Sites within Bound Regions of RNA-Binding Proteins

T Weirick, G Militello, MR Hosen, D John, JB Moore IV… - High-throughput, 2019 - mdpi.com
Studies in epitranscriptomics indicate that RNA is modified by a variety of enzymes. Among
these RNA modifications, adenosine to inosine (A-to-I) RNA editing occurs frequently in the …

[HTML][HTML] An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclear Drosophila head fractions

M Heim, L Blot, F Besse - STAR protocols, 2022 - Elsevier
RNA-binding proteins (RBPs) are multifunctional proteins that shuttle between the nucleus
and the cytoplasm where they assemble with target RNAs to form multi-molecular …

[HTML][HTML] Signaling pathways involved in the expression of SZNF and the target genes binding with SZNF related to cyadox

L Cui, D Xing, D Huang, D Li, Q Lu, X Wang… - Biomedicine & …, 2018 - Elsevier
SZNF (Sus scrofa zinc finger CCHC domain containing 3) is a post-transcription regulation
factor, belonging to CCHC zinc finger proteins. Cyadox is a novel quinoxaline drug with …