Strategy for the use of affinity grids to prepare non-His-tagged macromolecular complexes for single-particle electron microscopy
Affinity Grids are electron microscopy (EM) grids with a pre-deposited lipid monolayer
containing functionalized nickel-nitrilotriacetic acid lipids. Affinity Grids can be used to …
containing functionalized nickel-nitrilotriacetic acid lipids. Affinity Grids can be used to …
Targeted ferritinophagy in gastrointestinal cancer: from molecular mechanisms to implications
Z Feng, M Luan, W Zhu, Y Xing, X Ma, Y Wang… - Archives of …, 2024 - Springer
Gastrointestinal cancer is a significant global health burden, necessitating the development
of novel therapeutic strategies. Emerging evidence has highlighted the potential of targeting …
of novel therapeutic strategies. Emerging evidence has highlighted the potential of targeting …
[HTML][HTML] Eryptosis as a marker of Parkinson's disease
E Pretorius, AC Swanepoel, AV Buys… - Aging (Albany …, 2014 - ncbi.nlm.nih.gov
A major trend in recent Parkinson's disease (PD) research is the investigation of biological
markers that could help in identifying at-risk individuals or to track disease progression and …
markers that could help in identifying at-risk individuals or to track disease progression and …
Comparison of the binding behavior of FCCP with HSA and HTF as determined by spectroscopic and molecular modeling techniques
MM Moghaddam, M Pirouzi, MR Saberi… - …, 2014 - Wiley Online Library
The interaction of carbonylcyanide p‐(trifluoromethoxy) phenylhydrazone (FCCP) with
human serum albumin (HSA) and human transferrin (HTF) was investigated using multiple …
human serum albumin (HSA) and human transferrin (HTF) was investigated using multiple …
Structure prediction of transferrin receptor protein 1 (TfR1) by homology modelling, docking, and molecular dynamics simulation studies
MA Al-Refaei, RM Makki, HM Ali - Heliyon, 2020 - cell.com
Abstract Transferrin receptor protein 1 (TfR1) is an important molecule in anti-cancer
therapy. Targeted delivery of such therapeutic compounds improves their cellular uptake …
therapy. Targeted delivery of such therapeutic compounds improves their cellular uptake …
Nonfouling NTA-PEG-based TEM grid coatings for selective capture of histidine-tagged protein targets from cell lysates
We report the preparation and performance of TEM grids bearing stabilized nonfouling lipid
monolayer coatings. These films contain NTA capture ligands of controllable areal density at …
monolayer coatings. These films contain NTA capture ligands of controllable areal density at …
An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM
Q Zhao, X Hong, Y Wang, S Zhang, Z Ding… - Communications …, 2024 - nature.com
In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck,
particularly for rare or fragile macromolecular assemblies and those suffering from …
particularly for rare or fragile macromolecular assemblies and those suffering from …
Development of immobilized antibody-based affinity grid strategy and application in on-grid purification of target proteins for high-resolution cryo-EM
Q Zhao, X Hong, Y Wang, S Zhang, Z Ding, X Meng… - bioRxiv, 2024 - biorxiv.org
In cryo-electron microscopy (cryo-EM), sample preparation, especially for rare or fragile
macromolecular assemblies and those suffering from air-water interface denaturation and …
macromolecular assemblies and those suffering from air-water interface denaturation and …
A comparison of the inclusion behavior of human serum albumin and holo transferrin with fluoxymesterone in the presence of three different cyclodextrins
M Ghaderabad, M Mansouri, S Beigoli, AS Rad… - Journal of the Iranian …, 2017 - Springer
This article describes the interaction of fluoxymesterone (Flu) with HSA and HTF in the
absence and presence of cyclodextrins (CDs)(α, β and γ). According to fluorescence data …
absence and presence of cyclodextrins (CDs)(α, β and γ). According to fluorescence data …
[PDF][PDF] 生物导向药物研究进展
周天惠， 关婉怡， 赵宝华 - 中国医药生物技术, 2012 - cmbp.net.cn
生物导向药物(immunoconjugate) 又称生物靶向药物, 是有导向功能的载体与具有细胞杀伤能力
的物质的结合. 这类药物至少包括两个功能区: 一个功能区是载体(如单抗, 细胞因子等) …
的物质的结合. 这类药物至少包括两个功能区: 一个功能区是载体(如单抗, 细胞因子等) …