Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics

A Dorey, S Howorka - Nature chemistry, 2024 - nature.com
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid
readout, high accuracy, low cost and portability. This label-free method for sequencing at the …

Crossing epigenetic frontiers: the intersection of novel histone modifications and diseases

W Yao, X Hu, X Wang - Signal Transduction and Targeted Therapy, 2024 - nature.com
Histone post-translational modifications (HPTMs), as one of the core mechanisms of
epigenetic regulation, are garnering increasing attention due to their close association with …

Rapid species-level metagenome profiling and containment estimation with sylph

J Shaw, YW Yu - Nature Biotechnology, 2024 - nature.com
Profiling metagenomes against databases allows for the detection and quantification of
microorganisms, even at low abundances where assembly is not possible. We introduce …

Comparing genomes recovered from time-series metagenomes using long-and short-read sequencing technologies

LH Orellana, K Krüger, C Sidhu, R Amann - Microbiome, 2023 - Springer
Background Over the past years, sequencing technologies have expanded our ability to
examine novel microbial metabolisms and diversity previously obscured by isolation …

Production, formulation, and application of postbiotics in the treatment of skin conditions

A da Silva Vale, GV de Melo Pereira, AC de Oliveira… - Fermentation, 2023 - mdpi.com
The skin microbiome is composed of a complex association of bacteria, fungi, and viruses.
The maintenance of skin commensal microbes is essential for preventing the overgrowth of …

Reliability of species detection in 16S microbiome analysis: Comparison of five widely used pipelines and recommendations for a more standardized approach

A Hiergeist, J Ruelle, S Emler, A Gessner - PLoS One, 2023 - journals.plos.org
The use of NGS-based testing of the bacterial microbiota is often impeded by inconsistent or
non-reproducible results, especially when applying different analysis pipelines and …

Identification of dominant spoilage bacteria, bacterial diversity analysis, and antibacterial exploration of chilled monkfish

M Wang, Q Kong, H Yu, M Jiang, Z Han - Food Bioscience, 2023 - Elsevier
In this study, we aimed to investigate spoilage bacteria in monkfish stored under vacuum
and aerobic (air) refrigeration at 4° C for the first time. In vitro bacterial cultures, coupled with …

The role of oral microbiota in cancer

Z Lan, WJ Liu, H Cui, KL Zou, H Chen… - Frontiers in …, 2023 - frontiersin.org
Cancer remains a significant global challenge, with an estimated 47% increase in cancer
patients from 2020 to 2040. Increasing research has identified microorganism as a risk factor …

[HTML][HTML] TRP channels in cancer: Therapeutic opportunities and research strategies

J Xu, Z Wang, Y Niu, Y Tang, Y Wang, J Huang… - Pharmacological …, 2024 - Elsevier
The influence of gut microbiota on transient receptor potential (TRP) channels has been
identified as an important element in developing gastrointestinal conditions, yet its …

An integrated method for targeted Oxford Nanopore sequencing and automated bioinformatics for the simultaneous detection of bacteria, fungi, and ARG

S Kuruwa, A Zade, S Shah, R Moidu… - Journal of Applied …, 2024 - academic.oup.com
Aims The use of metagenomics for pathogen identification in clinical practice has been
limited. Here we describe a workflow to encourage the clinical utility and potential of NGS for …