Onsite detection of plant viruses using isothermal amplification assays

AI Bhat, R Aman, M Mahfouz - Plant biotechnology journal, 2022 - Wiley Online Library
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

Development and application of crude sap-based recombinase polymerase amplification assay for the detection and occurrence of grapevine geminivirus A in Indian …

G Kishan, R Kumar, SK Sharma, N Srivastava… - Frontiers in Plant …, 2023 - frontiersin.org
Geminiviruses are known to infect several fields and horticultural crops around the globe.
Grapevine geminivirus A (GGVA) was reported in the United States in 2017, and since then …

Development of Simplified Recombinase Polymerase Amplification Assay for Rapid and Robust Detection of Citrus Yellow Vein Clearing Virus

N Gupta, R Kumar, G Kishan, SK Sharma… - Current …, 2024 - Springer
Citrus is an economically important fruit crop, belongs to family Rutaceae, cultivated
commercially in over 130 countries, which holds a leading profitable position in the …

Trouble-free detection of grapevine leafroll-associated virus-3 employing reverse transcription-recombinase polymerase amplification assay

G Kishan, R Kumar, SK Sharma, N Srivastava… - Journal of Plant …, 2024 - Springer
Abstract Grapevine leafroll-associated virus-3 (GLRaV-3) is a viral pathogen of grapevine
leafroll disease (GLD) complex, responsible for debilitating viticulture across the globe. The …

Development of reverse transcription recombinase polymerase amplification assay for rapid diagnostics of Peanut mottle virus

B Parameswari, P Anbazhagan, A Rajashree… - … and Molecular Biology …, 2024 - Springer
Peanut mottle virus (PeMoV) is a single-stranded RNA virus transmitted through seeds and
aphids that affects peanut crops worldwide. Currently, Enzyme Linked Immune-Sorbent …

Light supplement by dark break shortens the growth period from bulblet to virus-free seed garlic

B Fan, Y Shang, J Han, B Cao, H Gao, X Liu, H Li… - Scientia …, 2022 - Elsevier
The garlic (Allium sativum L.) industry is faced with severe problems such as virus
accumulation and low reproduction coefficients due to long-term asexual propagation. In …

Gopi Kishan 1, 2, Rakesh Kumar1, Susheel Kumar Sharma1, Nishant Srivastava1, Nitika Gupta1, Ashwini Kumar1 and Virendra Kumar Baranwal1

G Kishan - Current advancements in real-time plant pathogen …, 2023 - books.google.com
Grape is one of the first and important fruit crops to be cultivated by man (McGovern, 2019).
Viruses are among the major biotic stressors for grapevine, and more than 80 viruses and …

Next-generation molecular diagnostics for plant viruses

A Srivastava, S Srivastava, T Gupta, N Rishi - Virus Diseases of …, 2021 - Springer
Viral disease is a major constraint for the yield loss in many economically important crops
and ultimately causes economic losses. Plant viruses are distributed from one infected crop …