Emerging clustered regularly interspaced short palindromic repeat/associated protein
(CRISPR/Cas) genome editing technology shows great potential in gene therapy. However …

As genome-editing nucleases move toward broader clinical applications, the need to define
the limits of their specificity and efficiency increases. A variety of approaches for nuclease …

The CRISPR effector Cas13 could be an effective antiviral for single-stranded RNA (ssRNA)
viruses because it programmably cleaves RNAs complementary to its CRISPR RNA …

Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of
plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV …

Despite numerous viral outbreaks in the last decade, including a devastating global
pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas …

Abstract Type V and VI CRISPR enzymes are RNA-guided, DNA and RNA-targeting
effectors that allow specific gene knockdown. Cas12 and Cas13 are CRISPR proteins that …

The recently discovered CRISPR-Cas gene editing system and its derivatives have found
numerous applications in fundamental biology research and pharmaceutical sciences. The …

Background A barrier to HIV-1 cure rests in the persistence of proviral DNA in infected CD4+
leukocytes. The high HIV-1 mutation rate leads to viral diversity, immune evasion, and …

RNA knockdown in vivo carries significant potential for disease modeling and therapies.
Despite the emerging approaches of CRISPR/Cas9-mediated permanent knock out of …

The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory
disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV …