A guide to light-sheet fluorescence microscopy for multiscale imaging

RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy

HR Ueda, HU Dodt, P Osten, MN Economo… - Neuron, 2020 - cell.com
Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …

Light-sheet microscopy: a tutorial

OE Olarte, J Andilla, EJ Gualda… - Advances in Optics and …, 2018 - opg.optica.org
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

Acousto-optic systems for advanced microscopy

M Duocastella, S Surdo, A Zunino… - Journal of Physics …, 2020 - iopscience.iop.org
Acoustic waves in an optical medium cause rapid periodic changes in the refraction index,
leading to diffraction effects. Such acoustically controlled diffraction can be used to …

Airy beam assisted NIR-II light-sheet microscopy

P Liu, T Shi, H Li, H Chen, Y Huang, H Ma, T Zhu… - Nano Today, 2022 - Elsevier
Abstract Near-infrared II (1000–1700 nm) three-dimensional (3D) microscopy addresses a
significant improvement on the penetration depth for bioimaging, but is challenging in high z …

Airy-beam tomographic microscopy

J Wang, X Hua, C Guo, W Liu, S Jia - Optica, 2020 - opg.optica.org
We introduce Airy-beam tomographic microscopy (ATM) for high-resolution, volumetric,
inertia-free imaging of biological specimens. The work exploits the highly adjustable Airy …

Strategies for volumetric imaging with a fluorescence microscope

J Mertz - Optica, 2019 - opg.optica.org
Three-dimensional fluorescence imaging has been a longstanding goal for microscopists,
made all the more challenging when aiming for a trifecta of resolution, speed, and field of …

Fast inertia-free volumetric light-sheet microscope

M Duocastella, G Sancataldo, P Saggau… - ACS …, 2017 - ACS Publications
Fast noninvasive three-dimensional (3D) imaging is crucial for quantitatively studying highly
dynamic events ranging from flow cytometry to developmental biology. Light-sheet …

Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy

S Quirin, N Vladimirov, CT Yang, DS Peterka, R Yuste… - Optics letters, 2016 - opg.optica.org
Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to
detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of …

SPIM-fluid: open source light-sheet based platform for high-throughput imaging

EJ Gualda, H Pereira, T Vale, MF Estrada… - Biomedical optics …, 2015 - opg.optica.org
Light sheet fluorescence microscopy has recently emerged as the technique of choice for
obtaining high quality 3D images of whole organisms/embryos with low photodamage and …