The CRISPR toolbox in medical mycology: State of the art and perspectives

F Morio, L Lombardi, G Butler - PLoS pathogens, 2020 - journals.plos.org
Fungal pathogens represent a major human threat affecting more than a billion people
worldwide. Invasive infections are on the rise, which is of considerable concern because …

CRISPR-Based Genetic Manipulation of Candida Species: Historical Perspectives and Current Approaches

D Uthayakumar, J Sharma, L Wensing… - Frontiers in Genome …, 2021 - frontiersin.org
The Candida genus encompasses a diverse group of ascomycete fungi that have captured
the attention of the scientific community, due to both their role in pathogenesis and emerging …

Paradigm Shift: Candida parapsilosis sensu stricto as the Most Prevalent Candida Species Isolated from Bloodstream Infections with Increasing Azole-Non …

I Franconi, C Rizzato, A Tavanti, M Falcone, A Lupetti - Journal of Fungi, 2023 - mdpi.com
Candidemia is the fourth most common healthcare-related bloodstream infection. In recent
years, incidence rates of Candida parapsilosis have been on the rise, with differences in …

Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species

L Lombardi, J Oliveira-Pacheco, G Butler - Msphere, 2019 - Am Soc Microbiol
Many Candida species that cause infection have diploid genomes and do not undergo
classical meiosis. The application of clustered regularly interspaced short palindromic …

Agglutinin-Like Sequence (ALS) Genes in the Candida parapsilosis Species Complex: Blurring the Boundaries Between Gene Families That Encode Cell-Wall …

SH Oh, B Smith, AN Miller, B Staker, C Fields… - Frontiers in …, 2019 - frontiersin.org
The agglutinin-like sequence (Als) proteins are best-characterized in Candida albicans and
known for their role in adhesion of the fungal cell to host and abiotic surfaces. ALS …

Using Genomics to Shape the Definition of the Agglutinin-Like Sequence (ALS) Family in the Saccharomycetales

SH Oh, K Schliep, A Isenhower… - Frontiers in Cellular …, 2021 - frontiersin.org
The Candida albicans agglutinin-like sequence (ALS) family is studied because of its
contribution to cell adhesion, fungal colonization, and polymicrobial biofilm formation. The …

Characterization of the Candida orthopsilosis agglutinin-like sequence (ALS) genes

L Lombardi, M Zoppo, C Rizzato, D Bottai… - PLoS …, 2019 - journals.plos.org
Agglutinin like sequence (Als) cell-wall proteins play a key role in adhesion and virulence of
Candida species. Compared to the well-characterized Candida albicans ALS genes, little is …

[HTML][HTML] CpALS4770 and CpALS4780 contribution to the virulence of Candida parapsilosis

M Zoppo, M Di Luca, M Franco, C Rizzato… - Microbiological …, 2020 - Elsevier
The ability of yeast to adhere to biotic and abiotic surfaces represents an essential trait
during the early stages of infection. Agglutinin-like sequence (Als) cell-wall proteins play a …

Advances in molecular tools and in vivo models for the study of human fungal pathogenesis

D Malavia, NAR Gow, J Usher - Microorganisms, 2020 - mdpi.com
Pathogenic fungi represent an increasing infectious disease threat to humans, especially
with an increasing challenge of antifungal drug resistance. Over the decades, numerous …

Genetic Manipulation as a Tool to Unravel Candida parapsilosis Species Complex Virulence and Drug Resistance: State of the Art

M Zoppo, N Poma, M Di Luca, D Bottai, A Tavanti - Journal of Fungi, 2021 - mdpi.com
An increase in the rate of isolation of Candida parapsilosis in the past decade, as well as
increased identification of azole-resistant strains are concerning, and require better …