High-resolution DNA melting analysis for simple and efficient molecular diagnostics
GH Reed, JO Kent, CT Wittwer - Pharmacogenomics, 2007 - Taylor & Francis
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …
sequence matching. The melting profile of a PCR product depends on its GC content, length …
The use of gold nanoparticles in diagnostics and detection
R Wilson - Chemical Society Reviews, 2008 - pubs.rsc.org
The widespread use of gold nanoparticles (GNPs) as labels in diagnostics and detection is
due to a unique combination of chemical and physical properties that allow biological …
due to a unique combination of chemical and physical properties that allow biological …
An ancient duplication of apple MYB transcription factors is responsible for novel red fruit-flesh phenotypes
Anthocyanin accumulation is coordinated in plants by a number of conserved transcription
factors. In apple (Malus× domestica), an R2R3 MYB transcription factor has been shown to …
factors. In apple (Malus× domestica), an R2R3 MYB transcription factor has been shown to …
High‐resolution DNA melting analysis: advancements and limitations
CT Wittwer - Human mutation, 2009 - Wiley Online Library
Recent advances in fluorescent dyes, methods, instruments and software for DNA melting
analysis have created versatile new tools for variant scanning and genotyping. High …
analysis have created versatile new tools for variant scanning and genotyping. High …
Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis
GH Reed, CT Wittwer - Clinical chemistry, 2004 - academic.oup.com
Background: Screening for heterozygous sequence changes in PCR products, also known
as “mutation scanning”, is an important tool for genetic research and clinical applications …
as “mutation scanning”, is an important tool for genetic research and clinical applications …
Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing
PCR is widely employed as the initial DNA amplification step for genetic testing. However, a
key limitation of PCR-based methods is the inability to selectively amplify low levels of …
key limitation of PCR-based methods is the inability to selectively amplify low levels of …
[PDF][PDF] 实时荧光定量PCR 技术的原理及其应用研究进展
赵焕英， 包金风 - 中国组织化学与细胞化学杂志, 2007 - yeec.com
自从1983 年Mullis 发明聚合酶链式反应(polymerasechainreaction, PCR) 以来, PCR
技术很快成为科研, 临床诊断的热点技术. 但是传统PCR 技术在应用中一是不能准确定量 …
技术很快成为科研, 临床诊断的热点技术. 但是传统PCR 技术在应用中一是不能准确定量 …
Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
Background Knockdown resistance (kdr) is a well-characterized mechanism of resistance to
pyrethroid insecticides in many insect species and is caused by point mutations of the …
pyrethroid insecticides in many insect species and is caused by point mutations of the …
PCR-based detection methods for single-nucleotide polymorphism or mutation: real-time PCR and its substantial contribution toward technological refinement
K Matsuda - Advances in clinical chemistry, 2017 - Elsevier
Single-nucleotide polymorphisms (SNPs) and single-nucleotide mutations result from the
substitution of only a single base. The SNP or mutation can be relevant to disease …
substitution of only a single base. The SNP or mutation can be relevant to disease …
High resolution melting applications for clinical laboratory medicine
M Erali, KV Voelkerding, CT Wittwer - Experimental and molecular …, 2008 - Elsevier
Separation of the two strands of DNA with heat (melting) is a fundamental property of DNA
that is conveniently monitored with fluorescence. Conventional melting is performed after …
that is conveniently monitored with fluorescence. Conventional melting is performed after …