System, device and method for electroporation of cells

TN Corso, H Craighead - US Patent 11,225,638, 2022 - Google Patents
(57) ABSTRACT A system, device and method for electroporation of living cells and the
introduction of selected molecules into the cells utilizes a fluidic system where living cells …

Disruption and field enabled delivery of compounds and compositions into cells

X Ding, AR Sharei, RS Langer, KF Jensen - US Patent 10,526,573, 2020 - Google Patents
(57) ABSTRACT A microfluidic system for causing perturbations in a cell membrane includes
(a) a microfluidic channel defining a lumen and configured such that a cell suspended in a …

Electrochemical liquid treatment apparatus

DV Cam, JF Ellers, BD Hill - US Patent 10,850,994, 2020 - Google Patents
Apparatus includes treatment chamber including at least one outlet for exit of
electrochemically treated liquid, and a liquid disperser having a plurality of liquid …

Delivery of biomolecules to immune cells

AR Sharei, S Mao, G Hartoularos, LIU Sophia… - US Patent …, 2021 - Google Patents
A method and device for preferentially delivering a compound such as an antigen to the
cytosol of an immune cell. The method comprises passing a cell suspension comprising the …

Delivery of materials to anucleate cells

AR Sharei, KF Jensen, JR Abshire… - US Patent 11,299,698, 2022 - Google Patents
The current subject matter includes methods, systems, articles, and techniques to deliver
material to anucleate cells, such as red blood cells. Using a rapid deformation based …

Selective delivery of material to cells

AR Sharei, VA Adalsteinsson, N Cho… - US Patent …, 2018 - Google Patents
Isolating or identifying a cell based on a physical property of said cell can include providing
a cell suspension; passing said suspension through a microfluidic channel that includes a …

Protein delivery in primary hematopoietic cells

A Marson, J Doudna, J Bluestone, K Schumann, S Lin - 2016 - Google Patents
WO2016123578A1 - Protein delivery in primary hematopoietic cells - Google Patents
WO2016123578A1 - Protein delivery in primary hematopoietic cells - Google Patents …

Electrochemical treatment methods

DV Cam, JF Ellers, BD Hill - US Patent 11,046,595, 2021 - Google Patents
Methods of treating a fluid mixture include performing a first treatment on the mixture with
electrochemically produced ions to separate an aqueous phase and a hydrophobic phase …

Gene editing through microfluidic delivery

AR Sharei, M Lajoie, KF Jensen… - US Patent 11,125,739, 2021 - Google Patents
Gene editing can be performed by introducing gene-editing components into a cell by
mechanical cell disruption. Related apparatus, systems, techniques, and articles are also …

Selective delivery of material to cells

AR Sharei, VA Adalsteinsson, N Cho… - US Patent …, 2020 - Google Patents
Isolating or identifying a cell based on a physical property of said cell can include providing
a cell suspension; passing said suspension through a microfluidic channel that includes a …