Serial femtosecond crystallography

TRM Barends, B Stauch, V Cherezov… - Nature reviews Methods …, 2022 - nature.com
With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

Towards an optimal sample delivery method for serial crystallography at XFEL

RKY Cheng - Crystals, 2020 - mdpi.com
The advent of the X-ray free electron laser (XFEL) in the last decade created the discipline of
serial crystallography but also the challenge of how crystal samples are delivered to X-ray …

Time-resolved serial femtosecond crystallography at the European XFEL

S Pandey, R Bean, T Sato, I Poudyal, J Bielecki… - Nature …, 2020 - nature.com
Abstract The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces
femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition …

Membrane protein megahertz crystallography at the European XFEL

C Gisriel, J Coe, R Letrun, OM Yefanov… - Nature …, 2019 - nature.com
The world's first superconducting megahertz repetition rate hard X-ray free-electron laser
(XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train …

[PDF][PDF] 3D printed devices and infrastructure for liquid sample delivery at the European XFEL

M Vakili, J Bielecki, J Knoška, F Otte… - Journal of …, 2022 - journals.iucr.org
The Sample Environment and Characterization (SEC) group of the European X-ray Free-
Electron Laser (EuXFEL) develops sample delivery systems for the various scientific …

Segmented flow generator for serial crystallography at the European X-ray free electron laser

A Echelmeier, J Cruz Villarreal… - Nature …, 2020 - nature.com
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows
structure determination of membrane proteins and time-resolved crystallography. Common …

Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme

D Doppler, M Sonker, A Egatz-Gomez, A Grieco… - Lab on a Chip, 2023 - pubs.rsc.org
Droplet injection strategies are a promising tool to reduce the large amount of sample
consumed in serial femtosecond crystallography (SFX) measurements at X-ray free electron …

[HTML][HTML] Microfluidic polyimide gas dynamic virtual nozzles for serial crystallography

M Vakili, R Vasireddi, PV Gwozdz… - Review of Scientific …, 2020 - pubs.aip.org
Free liquid jets are a common sample delivery method in serial femtosecond x-ray (SFX)
crystallography. Gas dynamic virtual nozzles (GDVNs) use an outer gas stream to focus a …

[PDF][PDF] Fixed-target serial crystallography at the Structural Biology Center

DA Sherrell, A Lavens, M Wilamowski… - Journal of …, 2022 - journals.iucr.org
Serial synchrotron crystallography enables the study of protein structures under
physiological temperature and reduced radiation damage by collection of data from …

Cyclic Olefin Copolymer-Based Fixed-Target Sample Delivery Device for Protein X-ray Crystallography

A Manna, M Sonker, D Koh, M Steiger… - Analytical …, 2024 - ACS Publications
Serial macromolecular X-ray crystallography plays an important role in elucidating protein
structures and consequently progressing the field of targeted therapeutics. The use of …