Serial femtosecond crystallography reveals that photoactivation in a fluorescent protein proceeds via the hula twist mechanism

A Fadini, CDM Hutchison, D Morozov… - Journal of the …, 2023 - ACS Publications
Chromophore cis/trans photoisomerization is a fundamental process in chemistry and in the
activation of many photosensitive proteins. A major task is understanding the effect of the …

Insight into the structural dynamics of light sensitive proteins from time-resolved crystallography and quantum chemical calculations

M Weik, T Domratcheva - Current Opinion in Structural Biology, 2022 - Elsevier
The structural dynamics underlying molecular mechanisms of light-sensitive proteins can be
studied by a variety of experimental and computational biophysical techniques. Here we …

It's a two‐way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes

EC Rodrigues, AC Stiel - FEBS letters, 2023 - Wiley Online Library
Chromophore‐bearing proteins that are (reversibly) altered after light illumination are major
functional components of nature. They gained considerable attention in the last decades …

Photophysical studies at cryogenic temperature reveal a novel photoswitching mechanism of rsEGFP2

AMR Mantovanelli, O Glushonkov… - Journal of the …, 2023 - ACS Publications
Single-molecule localization microscopy (SMLM) at cryogenic temperature opens new
avenues to investigate intact biological samples at the nanoscale and perform cryo …

Light-Induced Forward and Reverse Intersystem Crossing in Green Fluorescent Proteins at Cryogenic Temperatures

L Rane, J Wulffele, D Bourgeois… - The Journal of …, 2023 - ACS Publications
Combining fluorescence and phosphorescence kinetics, we characterize forward and
reverse intersystem crossing (FISC and RISC, respectively) between the singlet and triplet …

A Photoswitchable Solvatochromic Dye for Probing Membrane Ordering by RESOLFT Super‐resolution Microscopy

AT Frawley, KG Leslie, V Wycisk, S Galiani… - …, 2023 - Wiley Online Library
A switchable solvatochromic fluorescent dyad can be used to map ordering of lipids in
vesicle membranes at a resolution better than the diffraction limit. Combining a Nile Red …

Structural heterogeneity in a phototransformable fluorescent protein impacts its photochemical properties

A Maity, J Wulffelé, I Ayala, A Favier, V Adam… - Advanced …, 2024 - Wiley Online Library
Photoconvertible fluorescent proteins (PCFP) are important cellular markers in advanced
imaging modalities such as photoactivatable localization microscopy (PALM). However, their …

Reversibly switchable fluorescent proteins:“the fair switch project”

R Nifosì, B Storti, R Bizzarri - La Rivista del Nuovo Cimento, 2024 - Springer
Fluorescent proteins (FPs) have transformed cell biology through their use in fluorescence
microscopy, enabling precise labeling of proteins via genetic fusion. A key advancement is …

[PDF][PDF] Ultrafast Light-Matter and Quantum Dynamics

S Carbajo - 2023 - escholarship.org
UCLA Previously Published Works Page 1 UCLA UCLA Previously Published Works Title
Ultrafast Light-Matter and Quantum Dynamics Permalink https://escholarship.org/uc/item/7c2519zj …