Main conclusion While transgenic technology has heralded a new era in crop improvement,
several concerns have precluded their widespread acceptance. Alternative technologies …

TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …

Genome editing promises giant leaps forward in advancing biotechnology, agriculture, and
basic research. The process relies on the use of sequence specific nucleases (SSNs) to …

Targeted genomic regulation is a powerful approach to accelerate trait discovery and
development in agricultural biotechnology. Bacteria and archaea use clustered regularly …

The constant struggle between plants and microbes has driven the evolution of multiple
defense strategies in the host as well as offense strategies in the pathogen. To defend …

Transcription activator-like (TAL) effectors are repeat-containing proteins used by plant
pathogenic bacteria to manipulate host gene expression. Repeats are polymorphic and …

Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9‐associated
protein systems provide a powerful genetic manipulation tool that can drive plant research …

Precise studies of plant, animal and human genomes enable remarkable opportunities of
obtained data application in biotechnology and medicine. However, knowing nucleotide …

User-friendly tools for robust transcriptional activation of endogenous genes are highly
demanded in plants. We previously showed that a dCas9-VP64 system consisting of the …

Synthetic biology seeks to create new biological systems, including user-designed plants
and plant cells. These systems can be employed for a variety of purposes, ranging from …