Highlights•Synthetic biology provides new tools for the dynamic control of
metabolism.•Dynamic control is crucial to further the field of metabolic engineering.•Sensors …

Throughout the decades of its history, the advances in bacteria-based bio-industries have
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …

We engineered Escherichia coli to grow on CO2 and formic acid alone by introducing the
synthetic CO2 and formic acid assimilation pathway, expressing two formate dehydrogenase …

While there are several genome editing techniques available, few are suitable for dynamic
and simultaneous mutagenesis of arbitrary targeted sequences in prokaryotes. Here, to …

Escherichia coli gene expression knockdown using synthetic small RNA (sRNA) can be fine-
tuned by altering sRNA sequences to modulate target mRNA-binding ability, but this …

Cinnamaldehyde (CAD) derived from cinnamon bark has received much attention for its
potential as a nematicide and food additive. Previously, we have succeeded in developing …

White biotechnology is a term that is now often used to describe the implementation of
biotechnology in the industrial sphere. Biocatalysts (enzymes and microorganisms) are the …

Generation of multiple genomic alterations is currently a time consuming process. Here, a
method was established that enables highly efficient and simultaneous deletion of multiple …

A novel metabolic pathway was designed for the production of 3-aminopropionic acid (3-
AP), an important platform chemical for manufacturing acrylamide and acrylonitrile. Using a …

Escherichia coli was metabolically engineered to produce industrially important platform
chemicals, 3-hydroxypropionic acid (3-HP) and malonic acid (MA), through the β-alanine …