Function and evolution of nematode RNAi pathways
Selfish genetic elements, like transposable elements or viruses, are a threat to genomic
stability. A variety of processes, including small RNA-based RNA interference (RNAi)-like …
stability. A variety of processes, including small RNA-based RNA interference (RNAi)-like …
[HTML][HTML] Mechanisms of epigenetic regulation by C. elegans nuclear RNA interference pathways
U Seroussi, C Li, AE Sundby, TL Lee… - Seminars in Cell & …, 2022 - Elsevier
RNA interference (RNAi) is a highly conserved gene regulatory phenomenon whereby
Argonaute/small RNA (AGO/sRNA) complexes target transcripts by antisense …
Argonaute/small RNA (AGO/sRNA) complexes target transcripts by antisense …
Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans
Abstract The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been
successfully used for genome editing in a variety of organisms. Here, we report the use of …
successfully used for genome editing in a variety of organisms. Here, we report the use of …
The Nrde pathway mediates small-RNA-directed histone H3 lysine 27 trimethylation in Caenorhabditis elegans
Small-RNA-mediated chromatin modifications have been widely studied in plants and S.
pombe [1–3]. However, direct evidence of small-RNA-guided sequence-specific chromatin …
pombe [1–3]. However, direct evidence of small-RNA-guided sequence-specific chromatin …
A cytoplasmic Argonaute protein promotes the inheritance of RNAi
RNAi-elicited gene silencing is heritable and can persist for multiple generations after its
initial induction in C. elegans. However, the mechanism by which parental-acquired trait …
initial induction in C. elegans. However, the mechanism by which parental-acquired trait …
Germ granules govern small RNA inheritance
In C. elegans nematodes, components of liquid-like germ granules were shown to be
required for transgenerational small RNA inheritance. Surprisingly, we show here that …
required for transgenerational small RNA inheritance. Surprisingly, we show here that …
Epimutations driven by small RNAs arise frequently but most have limited duration in Caenorhabditis elegans
Epigenetic regulation involves changes in gene expression independent of DNA sequence
variation that are inherited through cell division. In addition to a fundamental role in cell …
variation that are inherited through cell division. In addition to a fundamental role in cell …
RdRP-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway
Expression of rRNA affects cell growth and proliferation, but mechanisms that modulate
rRNA levels are poorly understood. We conducted a genetic screen for factors that …
rRNA levels are poorly understood. We conducted a genetic screen for factors that …
Defining an optimal control for RNAi experiments with adult Schistosoma mansoni
MF Moescheid, O Puckelwaldt, M Beutler… - Scientific Reports, 2023 - nature.com
In parasites such as Schistosoma mansoni, gene knockdown by RNA interference (RNAi)
has become an indispensable tool for functional gene characterization. To distinguish target …
has become an indispensable tool for functional gene characterization. To distinguish target …
Small RNA pathways in the nematode Ascaris in the absence of piRNAs
MV Zagoskin, J Wang, AT Neff, GMB Veronezi… - Nature …, 2022 - nature.com
Small RNA pathways play key and diverse regulatory roles in C. elegans, but our
understanding of their conservation and contributions in other nematodes is limited. We …
understanding of their conservation and contributions in other nematodes is limited. We …