Ancient DNA analysis

L Orlando, R Allaby, P Skoglund… - Nature reviews …, 2021 - nature.com
Although the first ancient DNA molecules were extracted more than three decades ago, the
first ancient nuclear genomes could only be characterized after high-throughput sequencing …

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: Overview and recommendations

E Capo, C Giguet-Covex, A Rouillard, K Nota… - Quaternary, 2021 - mdpi.com
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and
aquatic biota is a rapidly advancing field in paleoecological research. Although largely …

Environmental DNA for wildlife biology and biodiversity monitoring

K Bohmann, A Evans, MTP Gilbert, GR Carvalho… - Trends in ecology & …, 2014 - cell.com
Extraction and identification of DNA from an environmental sample has proven noteworthy
recently in detecting and monitoring not only common species, but also those that are …

[HTML][HTML] Origin, dynamics, and implications of extracellular DNA pools in marine sediments

A Torti, MA Lever, BB Jørgensen - Marine genomics, 2015 - Elsevier
In marine sediments, DNA occurs both inside and outside living organisms. DNA not
enclosed in living cells may account for the largest fraction of total DNA, and include …

Extracellular DNA in natural environments: features, relevance and applications

M Nagler, H Insam, G Pietramellara… - Applied microbiology and …, 2018 - Springer
Extracellular DNA (exDNA) is abundant in many habitats, including soil, sediments, oceans
and freshwater as well as the intercellular milieu of metazoa. For a long time, its origin has …

Accurate multiplexing and filtering for high-throughput amplicon-sequencing

P Esling, F Lejzerowicz, J Pawlowski - Nucleic acids research, 2015 - academic.oup.com
Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …

Deep-sea, deep-sequencing: metabarcoding extracellular DNA from sediments of marine canyons

M Guardiola, MJ Uriz, P Taberlet, E Coissac… - PloS one, 2015 - journals.plos.org
Marine sediments are home to one of the richest species pools on Earth, but logistics and a
dearth of taxonomic work-force hinders the knowledge of their biodiversity. We characterized …

Worldwide analysis of sedimentary DNA reveals major gaps in taxonomic knowledge of deep-sea benthos

F Sinniger, J Pawlowski, S Harii, AJ Gooday… - Frontiers in Marine …, 2016 - frontiersin.org
Deep-sea sediments represent the largest but least known ecosystem on earth. With
increasing anthropogenic pressure, it is now a matter of urgency to improve our …

Protist metabarcoding and environmental biomonitoring: time for change

J Pawlowski, F Lejzerowicz… - European Journal of …, 2016 - Elsevier
High-throughput amplicon sequencing of environmental DNA and/or RNA proved to be a
powerful tool to describe protist diversity. This new approach called also the metabarcoding …