Electroporation designates the use of short high‐voltage pulses to overcome the barrier of
the cell membrane. By applying an external electric field, which just surpasses the …

Gene therapy is on its way to revolutionize the treatment of both inherited and acquired
diseases, by transferring nucleic acids to correct a disease-causing gene in the target cells …

Membrane electroporation is the method to directly transfer bioactive substances such as
drugs and genes into living cells, as well as preceding electrofusion. Although much …

Electroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be
used with most cell types, yields a high frequency of both stable transformation and transient …

Electroporation has been used extensively to transfer DNA to bacteria, yeast, and
mammalian cells in culture for the past 30 years. Over this time, numerous advances have …

In recent years, DNA vaccines have undergone a number of technological advancements
that have incited renewed interest and heightened promise in the field. Two such …

In many cases, nonviral particle-mediated gene delivery is highly dependent on the cell
cycle status of transfected cells. Here we compare particle-mediated delivery with linear …

It is generally recognized that DNA vaccines are often less effective in large animals than in
mice. One possible reason for this reduced effectiveness may be transfection efficiency and …

It is generally recognized that DNA vaccines are often less effective in large animals than in
mice. One possible reason for this reduced effectiveness may be transfection deficiency and …

Nonviral gene transfer is markedly enhanced by the application of in vivo electroporation
(also denoted electrogene transfer or electrokinetic enhancement). This approach is safe …