Quantitative mass spectrometry-based proteomics: an overview

S Rozanova, K Barkovits, M Nikolov, C Schmidt… - Quantitative methods in …, 2021 - Springer
In recent decades, mass spectrometry has moved more than ever before into the front line of
protein-centered research. After being established at the qualitative level, the more …

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

DP Donnelly, CM Rawlins, CJ DeHart, L Fornelli… - Nature …, 2019 - nature.com
One gene can give rise to many functionally distinct proteoforms, each of which has a
characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact …

Novel strategies to address the challenges in top-down proteomics

JA Melby, DS Roberts, EJ Larson… - Journal of the …, 2021 - ACS Publications
Top-down mass spectrometry (MS)-based proteomics is a powerful technology for
comprehensively characterizing proteoforms to decipher post-translational modifications …

Mass spectrometry using electrospray ionization

GRD Prabhu, ER Williams, M Wilm… - Nature Reviews Methods …, 2023 - nature.com
Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …

Decoding post-translational modification crosstalk with proteomics

M Leutert, SW Entwisle, J Villén - Molecular & Cellular Proteomics, 2021 - ASBMB
Post-translational modification (PTM) of proteins allows cells to regulate protein functions,
transduce signals and respond to perturbations. PTMs expand protein functionality and …

Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications

XR Liu, MM Zhang, ML Gross - Chemical reviews, 2020 - ACS Publications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Progress in top-down proteomics and the analysis of proteoforms

TK Toby, L Fornelli, NL Kelleher - Annual review of analytical …, 2016 - annualreviews.org
From a molecular perspective, enactors of function in biology are intact proteins that can be
variably modified at the genetic, transcriptional, or post-translational level. Over the past 30 …

[HTML][HTML] Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013)

KK Murray, RK Boyd, MN Eberlin… - Pure and Applied …, 2013 - degruyter.com
This document contains recommendations for terminology in mass spectrometry.
Development of standard terms dates back to 1974 when the IUPAC Commission on …

A statistical model for identifying proteins by tandem mass spectrometry

AI Nesvizhskii, A Keller, E Kolker… - Analytical …, 2003 - ACS Publications
A statistical model is presented for computing probabilities that proteins are present in a
sample on the basis of peptides assigned to tandem mass (MS/MS) spectra acquired from a …

Proteomics by mass spectrometry: approaches, advances, and applications

JR Yates, CI Ruse… - Annual review of …, 2009 - annualreviews.org
Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …