CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …

CRISPR systems for COVID-19 diagnosis

H Rahimi, M Salehiabar, M Barsbay, M Ghaffarlou… - ACS …, 2021 - ACS Publications
The emergence of the new coronavirus 2019 (COVID-19) was first seen in December 2019,
which has spread rapidly and become a global pandemic. The number of cases of COVID …

Smartphone-based SARS-CoV-2 and variants detection system using colorimetric DNAzyme reaction triggered by loop-mediated isothermal amplification (LAMP) with …

J Song, B Cha, J Moon, H Jang, S Kim, J Jang… - ACS …, 2022 - ACS Publications
Coronavirus disease (COVID-19) has affected people for over two years. Moreover, the
emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has …

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

Z Li, X Xu, D Wang, X Jiang - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Abstract The coronavirus disease 2019 (COVID-19) has extensively promoted the
application of nucleic acid testing technology in the field of clinical testing. The most widely …

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

SY Wang, YC Du, DX Wang, JY Ma, AN Tang… - Analytica Chimica …, 2021 - Elsevier
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) proteins are powerful gene-editing tools because of their ability to accurately …

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

X Zhang, Y Shi, G Chen, D Wu, Y Wu, G Li - Small Methods, 2022 - Wiley Online Library
Infectious pathogens cause severe human illnesses and great deaths per year worldwide.
Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing …

Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA

J Moon, C Liu - Nature Communications, 2023 - nature.com
Nucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and
deployable approach to molecular diagnostics. While exciting, there remain challenges …

Advances in clustered, regularly interspaced short palindromic repeats (CRISPR)-based diagnostic assays assisted by micro/nanotechnologies

H Yue, M Huang, T Tian, E Xiong, X Zhou - ACS nano, 2021 - ACS Publications
Clustered, regularly interspaced short palindromic repeats (CRISPR)-based diagnoses,
derived from gene-editing technology, have been exploited for less than 5 years and are …

Recent advances in airborne pathogen detection using optical and electrochemical biosensors

R Sivakumar, NY Lee - Analytica chimica acta, 2022 - Elsevier
The world is currently facing an adverse condition due to the pandemic of airborne pathogen
SARS-CoV-2. Prevention is better than cure; thus, the rapid detection of airborne pathogens …

Loop-mediated isothermal amplification-based electrochemical sensor for detecting SARS-CoV-2 in wastewater samples

RG Ramírez-Chavarría, E Castillo-Villanueva… - Journal of …, 2022 - Elsevier
The current pandemic COVID-19 caused by the coronavirus SARS-CoV-2, has generated
different economic, social and public health problems. Moreover, wastewater-based …