CENP-A nucleosome—a chromatin-embedded pedestal for the centromere: lessons learned from structural biology

A Ali-Ahmad, N Sekulić - Essays in Biochemistry, 2020 - portlandpress.com
The centromere is a chromosome locus that directs equal segregation of chromosomes
during cell division. A nucleosome containing the histone H3 variant CENP-A epigenetically …

Stabilization of the hexasome intermediate during histone exchange by yeast SWR1 complex

ASB Jalal, P Girvan, EYD Chua, L Liu, S Wang… - Molecular Cell, 2024 - cell.com
The yeast SWR1 complex catalyzes the exchange of histone H2A/H2B dimers in
nucleosomes with Htz1/H2B dimers. We use cryoelectron microscopy to determine the …

Molecular basis of global promoter sensing and nucleosome capture by the SWR1 chromatin remodeler

RK Louder, G Park, Z Ye, JS Cha, AM Gardner, Q Lei… - Cell, 2024 - cell.com
The SWR1 chromatin remodeling complex is recruited to+ 1 nucleosomes downstream of
transcription start sites of eukaryotic promoters, where it exchanges histone H2A for the …

Nucleosome remodelling: structural insights into ATP-dependent remodelling enzymes

R Sundaramoorthy - Essays in biochemistry, 2019 - portlandpress.com
ATP-dependent chromatin remodelling enzymes play a fundamental role in determining
how nucleosomes are organised, and render DNA sequences accessible to interacting …

TBP bookmarks and preserves neural stem cell fate memory by orchestrating local chromatin architecture

Y Shen, K Liu, J Liu, J Shen, T Ye, R Zhao, R Zhang… - Molecular Cell, 2024 - cell.com
Mitotic bookmarking has been posited as an important strategy for cells to faithfully
propagate their fate memory through cell generations. However, the physiological …

Mechanistic basis of gene regulation by SRCAP and H2A. Z

A Tollenaere, E Ugur, C Deluz, H Leonhardt, DM Suter - bioRxiv, 2024 - biorxiv.org
A central problem in gene regulation is to discriminate gene regulatory functions of
chromatin composition from the one of chromatin-modifying complexes. This question has …

[HTML][HTML] Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

M Ghoneim, CA Musselman - STAR protocols, 2023 - Elsevier
Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in
understanding the interaction of chromatin-associated factors with nucleosomes, the basic …