Ice thickness is a critical parameter in single particle cryo-EM–too thin ice can break during
imaging or exclude the sample of interest, while ice that is too thick contributes to more …

Single-particle cryo-electron microscopy (cryoEM) is a swiftly growing method for
understanding protein structure. With increasing demand for high-throughput, high …

In cryo-transmission electron microscopy (cryo-TEM), sample thickness is one of the most
important parameters that governs image quality. When combining cryo-TEM with other …

Advancements in cryo-electron microscopy (cryoEM) techniques over the past decade have
allowed structural biologists to routinely resolve macromolecular protein complexes to near …

CryoEM multi-grid screening is often a tedious process that demands hours of attention.
Here, this protocol shows how to set up standard Leginon collection and Smart Leginon …

The most widely used sample preparation method for single particle cryo-electron
microscopy (cryo-EM) today involves the application of 3-4 μl of sample onto a cryo-EM grid …

Sample preparation for cryo-EM imaging involves suspending macromolecules in a film of
vitreous ice that is simultaneously thin enough for electron transmission and thick enough to …

The chameleon is a cryo-EM sample preparation device (Figure 1) based on Spotiton [1].
Chameleon's automated workflow reduces user involvement, aims to produce consistent …

The Nipah virus (NiV) is a highly pathogenic RNA virus and a member of the
Paramyxoviridae family. Its genomic RNA is wrapped by the nucleocapsid (N) protein in a …