Methodological guidelines to study extracellular vesicles

FAW Coumans, AR Brisson, EI Buzas… - Circulation …, 2017 - Am Heart Assoc
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …

Biological functions and current advances in isolation and detection strategies for exosome nanovesicles

K Boriachek, MN Islam, A Möller, C Salomon… - Small, 2018 - Wiley Online Library
Abstract Exosomes are nanoscale (≈ 30–150 nm) extracellular vesicles of endocytic origin
that are shed by most types of cells and circulate in bodily fluids. Exosomes carry a specific …

Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics

B Dhondt, C Pinheiro, E Geeurickx… - Journal of …, 2023 - Wiley Online Library
The analysis of extracellular vesicles (EV) in blood samples is under intense investigation
and holds the potential to deliver clinically meaningful biomarkers for health and disease …

Advanced liquid biopsy technologies for circulating biomarker detection

N Soda, BHA Rehm, P Sonar, NT Nguyen… - Journal of Materials …, 2019 - pubs.rsc.org
Liquid biopsy is a new diagnostic concept that provides important information for monitoring
and identifying tumor genomes in body fluid samples. Detection of tumor origin …

[HTML][HTML] Impact of pre‐analytical parameters on the measurement of circulating microparticles: towards standardization of protocol

R Lacroix, C Judicone, P Poncelet, S Robert… - Journal of Thrombosis …, 2012 - Elsevier
Background: Microparticles (MP) are small vesicles of 0.1–1 μm, released in response to
activation or apoptosis. Over the past decade, they received an increasing interest both as …

Elevated levels of circulating platelet microparticles, VEGF, IL-6 and RANTES in patients with gastric cancer: possible role of a metastasis predictor

HK Kim, KS Song, YS Park, YH Kang, YJ Lee… - European journal of …, 2003 - Elsevier
The activation of coagulation, angiogenesis and inflammatory cytokines are considered to
be related with tumour growth and metastasis. We investigated the plasma levels of platelet …

Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges

K Singh, R Nalabotala, KM Koo, S Bose, R Nayak… - Analyst, 2021 - pubs.rsc.org
Exosomes are nano-sized extracellular vesicles that serve as a communications system
between cells and have shown tremendous promise as liquid biopsy biomarkers in …

Platelet microparticles induce angiogenesis in vitro

HK Kim, KS Song, JH Chung, KR Lee… - British journal of …, 2004 - Wiley Online Library
Platelet microparticles (PMP) are endogenous substances generated during the coagulation
process in a hypercoagulable state. This study demonstrated that PMP promote the …

Pre-analytical and analytical issues in the analysis of blood microparticles

Y Yuana, RM Bertina, S Osanto - Thrombosis and haemostasis, 2011 - thieme-connect.com
Results of plasma microparticles (MPs) measurements reported in the literature vary widely.
This is clearly not only related to the lack of well-standardised MP assays, but also to …

[HTML][HTML] Methodology for isolation, identification and characterization of microvesicles in peripheral blood

M Jayachandran, VM Miller, JA Heit… - Journal of immunological …, 2012 - Elsevier
RATIONALE: Analyses of circulating cell membrane-derived microvesicles (MV) have come
under scrutiny as potential diagnostic and prognostic biomarkers of disease. However …