Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …

Variants of uncertain significance fundamentally limit the clinical utility of genetic information.
The challenge they pose is epitomized by BRCA1, a tumour suppressor gene in which …

Recent advances in the development of genome editing technologies based on
programmable nucleases have substantially improved our ability to make precise changes …

It is extremely rare for a single experiment to be so impactful and timely that it shapes and
forecasts the experiments of the next decade. Here, we review how two such experiments …

The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated (Cas) system has emerged recently as a powerful method to manipulate the …

We and others recently demonstrated that the readily programmable CRISPR/Cas9 system
can be used to edit the Drosophila genome. However, most applications to date have relied …

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the
Drosophila genome and report that Cas9-mediated genomic modifications are efficiently …

Here, we present a simple and highly efficient method for generating and detecting
mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 …

Genome engineering with programmable nucleases depends on cellular responses to a
targeted double-strand break (DSB). The first truly targetable reagents were the zinc finger …