Glycoconjugates: synthesis, functional studies, and therapeutic developments

SS Shivatare, VS Shivatare, CH Wong - Chemical reviews, 2022 - ACS Publications
Glycoconjugates are major constituents of mammalian cells that are formed via covalent
conjugation of carbohydrates to other biomolecules like proteins and lipids and often …

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

M Karbalaei, SA Rezaee… - Journal of cellular …, 2020 - Wiley Online Library
One of the most important branches of genetic engineering is the expression of recombinant
proteins using biological expression systems. Nowadays, different expression systems are …

Recombinant protein vaccines, a proven approach against coronavirus pandemics

J Pollet, WH Chen, U Strych - Advanced drug delivery reviews, 2021 - Elsevier
With the COVID-19 pandemic now ongoing for close to a year, people all over the world are
still waiting for a vaccine to become available. The initial focus of accelerated global …

Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

P Patra, M Das, P Kundu, A Ghosh - Biotechnology Advances, 2021 - Elsevier
Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

M Ahmad, M Hirz, H Pichler, H Schwab - Applied microbiology and …, 2014 - Springer
Pichia pastoris is an established protein expression host mainly applied for the production of
biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished …

Yeast expression systems: overview and recent advances

R Baghban, S Farajnia, M Rajabibazl, Y Ghasemi… - Molecular …, 2019 - Springer
Yeasts are outstanding hosts for the production of functional recombinant proteins with
industrial or medical applications. Great attention has been emerged on yeast due to the …

Chemoenzymatic methods for the synthesis of glycoproteins

C Li, LX Wang - Chemical reviews, 2018 - ACS Publications
Glycosylation is one of the most prevalent posttranslational modifications that profoundly
affects the structure and functions of proteins in a wide variety of biological recognition …

Challenges and progress towards industrial recombinant protein production in yeasts: a review

P De Brabander, E Uitterhaegen, T Delmulle… - Biotechnology …, 2023 - Elsevier
Recombinant proteins (RP) are widely used as biopharmaceuticals, industrial enzymes, or
sustainable food source. Yeasts, with their ability to produce complex proteins through a …

Impact of host cell line choice on glycan profile

JB Goh, SK Ng - Critical reviews in biotechnology, 2018 - Taylor & Francis
Protein glycosylation is post-translational modification (PTM) which is important for
pharmacokinetics and immunogenicity of recombinant glycoprotein therapeutics. As a result …

[HTML][HTML] Cultivation strategies to enhance productivity of Pichia pastoris: a review

V Looser, B Bruhlmann, F Bumbak, C Stenger… - Biotechnology …, 2015 - Elsevier
Pichia pastoris, a methylotrophic yeast, is an established system for the production of
heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise …