Mass cytometry (cytometry by time-of-flight detection [CyTOF]) is a bioanalytical technique
that enables the identification and quantification of diverse features of cellular systems with …

These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

Regulatory T cells (Treg cells) can activate multiple suppressive mechanisms in vitro after
activation via the T cell antigen receptor, resulting in antigen-independent suppression …

B cells are activated by two temporally distinct signals, the first provided by the binding of
antigen to the B cell antigen receptor (BCR), and the second provided by helper T cells …

Dendritic cells (DCs) play a crucial role in initiating and shaping immune responses. The
effects of DCs on adaptive immune responses depend partly on functional specialization of …

Protective antibody responses to vaccination or infection depend on affinity maturation, a
process by which high-affinity germinal center (GC) B cells are selected on the basis of their …

NK cells utilize a large array of receptors to screen their surroundings for aberrant or virus‐
infected cells. Given the vast diversity of receptors expressed on NK cells we seek to identify …

Key events in T cell–dependent antibody responses, including affinity maturation, are
dependent on the B cell's presentation of antigen to helper T cells at critical checkpoints in …

Activation of CD4+ T cells to proliferate drives cells toward aerobic glycolysis for energy
production while using mitochondria primarily for macromolecular synthesis. In addition, the …