Over 520 different amino acid substitution variants have been previously identified in the
systematic screening of 91 human DNA repair genes for sequence variation. Two algorithms …

We report here the identification of a cell line containing single and double missense
mutations in cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA …

Ligand-dependent activity has been engineered into enzymes for purposes ranging from
controlling cell morphology to reprogramming cellular signaling pathways. Where these …

The ability to selectively activate function of particular proteins via pharmacological agents is
a longstanding goal in chemical biology. Recently, we reported an approach for designing a …

Cavities and clefts are frequently important sites of interaction between natural enzymes or
receptors and their corresponding substrate or ligand molecules and exemplify the types of …

Most proteins contain small cavities that can be filled by replacing cavity-lining residues by
larger ones. Since shortening mutations in hydrophobic cores tend to destabilize proteins, it …

Although core residues can sometimes be replaced by shorter ones without introducing
significant changes in protein structure, the energetic consequences are typically large and …

Structural plasticity of mammalian cytochromes P450 (CYP) has recently been explored in
our laboratory and elsewhere to understand the ligand-binding promiscuity. CYP2B4 …

Abstract β‐lactamases confer antibiotic resistance, one of the most serious world‐wide
health problems, and are an excellent theoretical and experimental model in the study of …

Proteins could be used to carry and deliver small compounds. As a tool for designing ligand
binding sites in protein cores, a three-step virtual screening method is presented that has …