INTRODUCTION Protein-protein interactions play critical roles in biology, but the structures
of many eukaryotic protein complexes are unknown, and there are likely many interactions …

Glycosylphosphatidylinositol (GPI) molecules are complex glycophospholipids and serve as
membrane anchors for tethering many proteins to the cell surface. Attaching GPI to the …

Abstract Glycosylphosphatidylinositol (GPI)‐anchored proteins (GPI‐APs) are present
ubiquitously at the cell surface in all eukaryotes. They play a crucial role in the interaction of …

Eukaryotic cells are coated with an abundance of glycosylphosphatidylinositol anchor
proteins (GPI-APs) that play crucial roles in fertilization, neurogenesis, and immunity. The …

N-Glycosylation (NG) and disulfide bonds (DBs) are two prevalent co/post-translational
modifications (PTMs) that are often conserved and coexist in membrane and secreted …

Aspergillus niger has the ability to produce a large variety of proteases, which are of
particular importance for protein digestion, intracellular protein turnover, cell signaling …

Glycosylphosphatidylinositol (GPI) anchoring of proteins is a eukaryotic, post-translational
modification catalyzed by GPI transamidase (GPI-T). The Saccharomyces cerevisiae GPI-T …

Protein-protein interactions play critical roles in biology, but despite decades of effort, the
structures of many eukaryotic protein complexes are unknown, and there are likely many …

Glycosylphosphatidylinositol (GPI) anchoring of proteins is a ubiquitous posttranslational
modification in eukaryotic cells. GPI-anchored proteins (GPI-APs) play critical roles in …

Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the post-translational addition
of the GPI anchor to the C-terminus of some proteins. In most eukaryotes, Gpi8, the active …