Transcriptional regulation is at the heart of biological functions such as adaptation to a
changing environment or to new carbon sources. One of the mechanisms which has been …

The bacterial insertion sequence, IS911, is a member of the large IS3family. It transposes
using a mechanism known as Copy‐out–Paste‐in. This is a major transposition pathway as …

In many cases, transcriptional regulation involves the binding of transcription factors at sites
on the DNA that are not immediately adjacent to the promoter of interest. This action at a …

At many promoters, transcription is regulated by simultaneous binding of a protein to
multiple sites on DNA, but the structures and dynamics of such transcription factor-mediated …

We describe a tethered multifluorophore motion assay based on DNA origami for revealing
bimolecular reaction kinetics on the single-molecule level. Molecular binding partners may …

The maintenance of intact genetic information, as well as the deployment of transcription for
specific sets of genes, critically rely on a family of proteins interacting with DNA and …

Tethered particle motion (TPM) monitors the variations in the effective length of a single DNA
molecule by tracking the Brownian motion of a bead tethered to a support by the DNA …

Argonaute (Ago) proteins are found in all three domains of life. The best-characterized group
is eukaryotic Argonautes (eAgos), which are the core of RNA interference. The best …

Abstract Tethered Particle Motion (TPM) is a versatile in vitro technique for monitoring the
conformations a linear macromolecule, such as DNA, can exhibit. The technique involves …

Atomic force microscopy (AFM) allows the study of single protein–DNA interactions such as
those observed with the Type I Restriction–Modification systems. The mechanisms …