CRISPR/Cas9 technology has become the most examined gene editing technology in
recent years due to its simple design, yet low cost, high efficiency, and simple operation …

After decades of research, our knowledge of the complexity of cancer mechanisms,
elegantly summarized as 'hallmarks of cancer', is expanding, as are the therapeutic …

Melting and fast cooling double stranded DNA donor molecules prior to injection
dramatically increases the frequency of homology-directed repair for edits such as insertions …

In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to
generate germline edits. The remarkable ease of C. elegans germline editing is attributed to …

A growing body of work suggests that the material properties of biomolecular condensates
ensuing from liquid–liquid phase separation change with time. How this aging process is …

The requirement for Cas nucleases to recognize a specific PAM is a major restriction for
genome editing. SpCas9 variants SpG and SpRY, recognizing NGN and NRN PAMs …

The nuclear envelope, which protects and organizes the genome, is dismantled during
mitosis. In the Caenorhabditis elegans zygote, nuclear envelope breakdown (NEBD) of the …

A repetitive gait cycle is an archetypical component within the behavioral repertoire of many
animals including humans. It originates from mechanical feedback within proprioceptors to …

Nucleoporins (Nups) assemble nuclear pores that form the permeability barrier between
nucleoplasm and cytoplasm. Nucleoporins also localize in cytoplasmic foci proposed to …

Animals rely on chemosensory cues to survive in pathogen-rich environments. In
Caenorhabditis elegans, pathogenic bacteria trigger aversive behaviors through neuronal …