Initially, gene therapy was viewed as an approach for treating hereditary diseases, but its
potential role in the treatment of acquired diseases such as cancer is now widely …

Liposomes (phospholipid bilayer vesicles) are versatile and robust delivery systems for
induction of antibody and T lymphocyte responses to associated subunit antigens. In the last …

RNAi technology has brought a new category of treatments for various diseases including
genetic diseases, viral diseases, and cancer. Despite the great versatility of RNAi that can …

The use of various synthetic lipids and polymers to deliver DNA for gene therapy
applications has been the subject of intense examination for the last 15 years. Our …

Chitosans of defined molecular weight (Mw 10–213 kDa) and degree of deacetylation (DD
46–88%) were synthesized, complexed with pEGFP-C2 plasmid into nanoparticles (NP) and …

Background Systemic administration of linear polyethylenimine–DNA complexes (L-
PEI/DNA) results in transient expression of the transgene in the lung. This study analyzes …

Novel biocompatible polymeric gene carriers have been examined for their potential in
treating various genetic and acquired diseases. The use of polymeric gene carriers may …

The ability to deliver nucleic acids (eg, plasmid DNA, antisense oligonucleotides, siRNA)
offers the potential to develop potent vaccines and novel therapeutics. However, nucleic …

Cationic lipids are positively charged amphiphilic molecules which, for most of them, form
positively charged liposomes, sometimes in combination with a neutral helper lipid. Such …

Complexing TLR9 agonists such as plasmid DNA to cationic liposomes markedly
potentiates their ability to activate innate immunity. We therefore reasoned that liposomes …